B8895
Anti-Rabbit IgG (whole molecule)–Biotin antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(2)
About This Item
Empfohlene Produkte
Biologische Quelle
goat
Qualitätsniveau
Konjugat
biotin conjugate
Antikörperform
affinity isolated antibody
Antikörper-Produkttyp
secondary antibodies
Klon
polyclonal
Form
buffered aqueous solution
Methode(n)
direct ELISA: 1:200,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800
western blot: 1:400,000-1:800,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)
Lagertemp.
−20°C
Posttranslationale Modifikation Target
unmodified
Suchen Sie nach ähnlichen Produkten? Aufrufen Leitfaden zum Produktvergleich
Verwandte Kategorien
Allgemeine Beschreibung
IgG antibodies regulate several functions such as phagocytosis and complement activation. Thus they play a crucial role in facilitating cytological immune responses. Anti-rabbit IgG (whole molecule)–biotin antibody can be used in immunohistochemistry. The product shows minimal interspecies cross reactivity to human IgG hence can be used in enzyme immunoassays or dot blot in presence of human IgG. Anti-rabbit IgG (whole molecule)–biotin antibody reacts specifically to all rabbit immunoglobulin but has no reactivity with human IgG.
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins
Anti-Rabbit IgG (whole molecule)-Biotin antibody reacts with rabbit serum and rabbit IgG. The product does not cross-react with human IgG.
Anti-Rabbit IgG (whole molecule)-Biotin antibody reacts with rabbit serum and rabbit IgG. The product does not cross-react with human IgG.
Spezifität
Binds all rabbit Igs.
Immunogen
Purified rabbit IgG
Anwendung
Anti-Rabbit IgG (whole molecule)-Biotin antibody has been used in
- Immunofluorescence
- Immunohistochemistry
- Western blot
- Direct enzyme linked immunosorbent assay (ELISA)
Biochem./physiol. Wirkung
Avidin, Streptavidin or ExtrAvidine show strong affinity interaction with biotin rendering the biotin-avidin system an extremely effective tool in molecular biology, protein chemistry and immunology. The high specificity of the biotinylated antibody to rabbit IgG and the stability of the biotin-avidin complex has been exploited in variety of reagents (Avidin, Streptavidin or ExtrAvidin conjugated to FITC, TRITC, Peroxidase or Alkaline Phosphatase), for the detection and quantitation of rabbit IgG.
Sonstige Hinweise
Antibody adsorbed with human IgG.
Physikalische Form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide
Angaben zur Herstellung
Adsorbed to reduce background staining with human samples.
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Produkt-Auswahlhilfe.
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Kunden haben sich ebenfalls angesehen
Keigo Nakamura et al.
PloS one, 12(5), e0178442-e0178442 (2017-05-26)
Immunological tolerance between fetal allograft and mother is crucial for pregnancy establishment and maintenance; however, these mechanisms particularly those during the latter part of pregnancy have not been definitively elucidated. The aim of this study was to examine the presence
Metal ion chelation-based color generation for alkaline phosphatase-linked high-performance visual immunoassays
<BIG>Lei L, et al.</BIG>
Sensors and Actuators B, Chemical, 273, 35-40 (2018)
Leonurine prevents atherosclerosis via promoting the expression of ABCA1 and ABCG1 in a Ppargamma/Lxralpha signaling pathway-dependent manner
<BIG>Jiang T, et al.</BIG>
Cellular Physiology and Biochemistry, 43, 1703-1717 (2017)
Feng Yang et al.
Cancer research, 65(19), 8887-8895 (2005-10-06)
Our previous studies have defined reactive stroma in human prostate cancer and have developed the differential reactive stroma (DRS) xenograft model to evaluate mechanisms of how reactive stroma promotes carcinoma tumorigenesis. Analysis of several normal human prostate stromal cell lines
Francisco Delgado-Rosas et al.
Reproduction (Cambridge, England), 142(5), 745-755 (2011-08-25)
Implantation of a retrogradely shed endometrium during menstruation requires an adequate blood supply, which allows the growth of endometriotic lesions. This suggests that the development of endometriosis can be impaired by inhibiting angiogenesis. The growth of endometriotic foci is impaired
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.