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B7401

Sigma-Aldrich

Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL-Nummer:
MFCD00162627
UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

goat

Qualitätsniveau

200

Konjugat

biotin conjugate

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

secondary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Methode(n)

direct ELISA: 1:160,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400
western blot (chemiluminescent): 1:300,000 using total cell extract of HeLa cells (5-10 μg/well)

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

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Allgemeine Beschreibung

Goat polyclonal anti-Mouse IgG (Fc specific)–Biotin reacts with mouse IgG and mouse IgG, Fc fragment. It shows no reactivity with mouse IgG Fab fragment, human IgG, IgA, IgM, kappa and lambda light chain, bovine IgG and IgM, or horse IgG.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Biotin:avidin second antibody signal systems involve the conjugation of biotin to a secondary antibody. Once the secondary antibody binds to a target primary antibody in an immunochemical assay, the signal is developed by allowing avidin which has been conjugated to an enzyme or signal molecule to bind to the antibody associated biotin molecule.

Anwendung

Anti-Mouse IgG (Fc specific)−Biotin antibody produced in goat has been used in ELISA and quantitative suspension array technology (qSAT) multiplex antibody assay.
Goat polyclonal anti-Mouse IgG (Fc specific)–Biotin may be used to detect mouse IgG within serum or other biological systems. The antibody may be used as a secondary antibody in immunochemical assays based on biotin:avidin second antibody signal systems wherein mouse IgG is the primary antibody.

Sonstige Hinweise

Antibody adsorbed with bovine, equine and human serum proteins.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Angaben zur Herstellung

Adsorbed to reduce background staining with bovine, horse or human samples.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Rulan Bai et al.
Biology of reproduction, 93(2), 46-46 (2015-07-03)
A successful pregnancy depends on the blastocyst's implantation to the maternal endometrium; however, the initial interaction between blastocyst and uterine epithelium has not been well characterized. The objectives of this study were to determine if selectins and their ligands were
Optimization of incubation conditions of Plasmodium falciparum antibody multiplex assays to measure IgG, IgG 1--4, IgM and IgE using standard and customized reference pools for sero-epidemiological and vaccine studies
Ubillos I, et al.
Malaria Journal, 17(1), 219-219 (2018)
Impaired ICOSL in human myeloid dendritic cells promotes Th2 responses in patients with allergic rhinitis and asthma.
Shen C, Hupin C, Froidure A, et al.
Clinical and Experimental Allergy, 44(6), 831-841 (2014)
Adjuvant dependence of APS pathology-related low-affinity antibodies during secondary immune response to tetanus toxoid in BALB/c mice
Zivkovic
Immunologic Research, 56(1), 143-149 (2013)
The context of tetanus toxoid application influences the outcome of antigen-specific and self-directed humoral immune response
Stojanovic M, et al.
Microbiology and Immunology, 53(2), 89-100 (2009)
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