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B0529

Sigma-Aldrich

Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL-Nummer:
MFCD00162621
UNSPSC-Code:
12352203

Biologische Quelle

goat

Qualitätsniveau

200

Konjugat

biotin conjugate

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

secondary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Speziesreaktivität

mouse

Methode(n)

direct ELISA: 1:200,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300
western blot: 1:300,000-1:600,000 using using Hela cells extract

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

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Allgemeine Beschreibung

Binds all mouse Igs.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Each immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

Anwendung

Anti-Mouse IgG (Fab specific)-Biotin antibody produced in goat has been used in immunohistochemistry and immunofluorescence studies.
Anti-Mouse IgG (Fab specific)-Biotin antibody produced in goat is suitable for direct ELISA at a dilution of 1:200000 and for immunohistochemistry at a dilution of 1:300. The antibody was used to stain paraffin-embedded, rat skin, duodenum, esophagus and spleen sections at a dilution of 1:200. For immunofluorescence of Human Gingival Fibroblasts, a dilution of 1:200 was used. For immunoblotting experiments using mouse adipose tissue, antibody dilution of 1:30000 was used.

Biochem./physiol. Wirkung

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Sonstige Hinweise

Antibody adsorbed with human IgG and rat serum proteins.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Angaben zur Herstellung

Adsorbed to reduce background staining with human or rat samples.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Endharti AT, et al.
BMC Complementary and Alternative Medicine, 16(1), 374-374 (2016)
The Laboratory Rat (1998)
Targeted disruption of fibronectin-integrin interactions in human gingival fibroblasts by the RI protease ofPorphyromonas gingivalis W50
Scragg MA, et al.
Infection and Immunity, 67(4), 1837-1843 (1999)
Margaret A Scragg et al.
Infection and immunity, 70(10), 5740-5750 (2002-09-14)
Porphyromonas gingivalis is an important pathogen associated with destructive periodontal disease and is able to invade the epithelial cell barrier. Its cysteine proteases are recognized as major virulence factors, and in this study, we examined the interaction of the arginine-specific
Molecular Genetics of Immunoglobulin, 17 (1987)
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