58149-U
SUPELCOSIL™ LC-8-DB HPLC Column
3 μm particle size, L × I.D. 3.3 cm × 2.1 mm
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About This Item
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material
stainless steel column
Quality Level
agency
suitable for USP L7
product line
SUPELCOSIL™
feature
endcapped
manufacturer/tradename
SUPELCOSIL™
packaging
1 ea of
extent of labeling
6.0% carbon loading
parameter
0-70 °C temperature
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
3.3 cm × 2.1 mm
surface area
170 m2/g
surface coverage
surface coverage 3.2 μmol/m2
matrix
silica gel, spherical particle platform
fully porous particle
matrix active group
C8 (octyl) phase
particle size
3 μm
pore size
120 Å
operating pH
2-7.5
application(s)
food and beverages
separation technique
reversed phase
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General description
SUPELCOSIL LC-DB phases are specially deactivated for basic compounds. These columns provide less retention, better peak shape, and higher efficiency for organic bases than can be obtained on conventional reversed-phase columns.
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
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Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(1), 1-5 (2004-04-29)
A high-performance liquid chromatographic method is described for determination of lidocaine (2-(dietyloamino)-N-(2,6-dimetylofenylo) acetamid) and its metabolite, monoethylglycine xylidide (MEGX), in human serum containing various concentration of bilirubin. Lidocaine and its metabolite were extracted from human serum using dichloromethane. After separation
Journal of chromatography. B, Biomedical applications, 657(1), 83-92 (1994-07-01)
The isocratic, reversed-phase, high-performance liquid chromatographic (HPLC) method presented provides a simple and rapid analytical technique for the simultaneous determination of low-molecular-mass oligomers of polyethylene glycol (PEG) in aqueous polymer samples containing polar contaminants of biologic origin. In the present
Journal of pharmaceutical and biomedical analysis, 26(2), 179-187 (2001-07-27)
A reversed-phase high-performance liquid chromatographic method with coulometric and UV detection has been developed for the simultaneous determination of morphine, morphine-3-glucuronide and morphine-6-glucuronide. The separation was carried out by using a Supelcosil LC-8 DB reversed-phase column and 0.1 M potassium
Journal of chromatography. B, Biomedical applications, 665(2), 404-409 (1995-03-24)
A simple HPLC method has been developed for the determination of ticlopidine in human plasma. Plasma samples were buffered at pH 9 and extracted with n-heptane-isoamyl alcohol (98.5:1.5, v/v). Imipramine was used as internal standard. Chromatography was performed isocratically with
Clinical chemistry, 40(7 Pt 1), 1312-1316 (1994-07-01)
This automated assay determines the concentration of the antidepressant fluoxetine (Prozac) and its active metabolite norfluoxetine in serum by reversed-phase HPLC with spectrophotometric detection. Extraction, injection, and quantification are performed by the Gilson Aspec automated sample handler. The patient's specimen
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