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58355-U

Supelco

SUPELCOSIL™ LC-18-DB (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

Synonym(s):

High Purity C18 HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

SUPELCOSIL™ LC-18-DB HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

material

stainless steel column

Quality Level

100

agency

suitable for USP L1

product line

SUPELCOSIL™

feature

endcapped

manufacturer/tradename

SUPELCOSIL™

packaging

1 ea of

extent of labeling

11.0% Carbon loading

parameter

0-70 °C temperature
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

170 m2/g

surface coverage

surface coverage 3.1 μmol/m2

matrix

silica gel, spherical particle platform
fully porous particle

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

120 Å

operating pH

2-7.5

application(s)

food and beverages

compatibility

for use with PTC-AA

separation technique

reversed phase

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General description

SUPELCOSIL LC-DB phases are specially deactivated for basic compounds. These columns provide shorter retention, better peak shape, and higher efficiency for organic bases than can be obtained on other Type A silica reversed-phase columns.

Application


  • OPTIMIZATION AND VALIDATION OF HPLC METHOD FOR TETRAMETHRIN DETERMINATION IN HUMAN SHAMPOO FORMULATION.: This study discusses the optimization and validation of a high-performance liquid chromatography (HPLC) method using SUPELCOSIL™ LC-18-DB HPLC Column for the analysis of tetramethrin in shampoo formulations (Zeric Stosic et al., 2016).

  • Mass spectrometric characterization and HPLC determination of the main urinary metabolites of nimesulide in man.: Details a robust approach for identifying and quantifying nimesulide metabolites via HPLC, underscoring the column′s applicability in complex biological matrices (Carini et al., 1998).


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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Xinghua Sun et al.
Anticancer research, 25(1A), 59-62 (2005-04-09)
A rapid, sensitive and specific high-performance liquid chromatographic (HPLC) method for the separation and quantification of L-methionine in plasma has been developed. After derivatization of plasma amino acids with o-phthalaldehyde (OPA), a 50 microl sample was loaded on a reversed-phase
M Carini et al.
Journal of pharmaceutical and biomedical analysis, 18(1-2), 201-211 (1998-12-24)
A study was undertaken for the characterization and quantitative determination of the main urinary metabolites of the non-steroidal anti-inflammatory drug (NSAID) nimesulide (4-nitro-2-phenoxy-methanesulfonanilide) in man following single oral administration (200 mg). Urines were collected from six healthy volunteers at 12
P A Hynning et al.
Clinical chemistry, 34(12), 2502-2503 (1988-12-01)
A high-performance liquid chromatographic (HPLC) method for determining verapamil and norverapamil in plasma is presented and compared with gas chromatography/mass spectrometry (GC-MS). The plasma samples were extracted at alkaline pH with hexane containing 2-butanol (20 mL/L) and then back-extracted into
D C Holland et al.
Journal of AOAC International, 78(4), 1067-1071 (1995-07-01)
A liquid chromatographic (LC) method is described for the simultaneous determination of the triazine herbicides, simazine (SIM), atrazine (ATZ), and propazine (PRO) in the 12.5-100 ppb range in catfish. The herbicides are extracted from catfish homogenates with ethyl acetate, followed
O Majid et al.
Therapeutic drug monitoring, 23(2), 163-168 (2001-04-11)
Recipients of organ transplants remain particularly dependent on prednisolone as part of their maintenance immunosuppression. Despite this, the pharmacokinetics of prednisolone have never been fully characterized in these patients, and consequently dosing remains empirical. Accurate monitoring of prednisolone, its primary
View All Related Papers

Protocols

Deriviatization, Hydrolysis, and HPLC Analysis of Amino Acids on SUPELCOSIL™ and ASCENTIS® Columns

Amino Acid Analysis is the suitable tool for precise determination of protein quantities but also provides detailed information regarding the relative amino.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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