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T9378

Sigma-Aldrich

Trypsin inhibitor

crude powder

Synonym(s):

Trypsin Inhibitor from lima beans

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About This Item

CAS Number:
9035-81-8
EC Number:
232-906-9
MDL number:
MFCD00082102
UNSPSC Code:
12352202
NACRES:
NA.77

product name

Trypsin inhibitor from Phaseolus limensis (lima bean), Type II-L, crude powder

biological source

Phaseolus limensis (lima bean)

Quality Level

200

type

Type II-L

form

crude powder

mol wt

9 kDa

solubility

0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL

storage temp.

2-8°C

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Application

Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:
  • in the inhibition of trypsin in human plasma
  • in the termination of proteolytic digestion in rabbit skeletal muscles
  • in the inhibition of salivary glands enzyme extract from Lygus lineolaris
  • in the inhibition of proteolytic activity in Agave tequilana enzyme extract

Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type enzymes.

Biochem/physiol Actions

Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors. It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.

Physical properties

Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.

Unit Definition

One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Preparation Note

Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).

Analysis Note

One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Other Notes

View more information on Trypsin Inhibitor.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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G Bellon et al.
Artery, 7(4), 290-302 (1980-01-01)
A serine protease active on insoluble elastin at neutral pH has been isolated from human aortic media employing a Lima-bean trypsin inhibitor - Sepharose column. It is also hydrolyzed Suc (Ala)3 pna and casein but was found inactive against Benzoyl-Tyr-pna
In-house phase determination of the lima bean trypsin inhibitor: a low-resolution sulfur-SAD case
Debreczeni J, et al.
Acta crystallographica. Section D, Structural biology, 59(2), 393-395 (2003)
Are cardiovascular and sympathoadrenal effects of human ?new pressor protein? preparations attributable to human coagulation beta-FXIIa?
Papageorgiou PC, et al.
American Journal of Physiology. Heart and Circulatory Physiology, 286(3), H837-H846 (2004)
A Rapid and Reliable Method for Total Protein Extraction from Succulent Plants for Proteomic Analysis
Lledias F, et al.
The Protein Journal, 36(4), 308-321 (2017)
Shark skeletal muscle tropomyosin is a phosphoprotein
Hayley M, et al.
Journal of Muscle Research and Cell Motility, 29(2-5), 101-107 (2008)
View All Related Papers

Articles

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

Protocols

Enzymatic Assay of Trypsin Inhibitor

This technical article described the Enzymatic Assay of Trypsin Inhibitor.

Related Content

Trypsin Serine Protease Enzyme

Trypsin is an enzyme in the serine protease class that consists of a polypeptide chain of 223 amino acid residues. Multiple sources, grades and formulations of trypsin specifically designed for research applications are available.

Chromatograms

application for HPLC

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