GE28-9365-51
Hisprep™ Fast Flow 16/10
Cytiva 28-9365-51
About This Item
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shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9365-51
parameter
<10 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
bed size
16 mm × 100 mm
bed volume
20 mL
column I.D.
16 mm
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
capacity
~40 mg binding capacity(histidine-tagged protein)
suitability
suitable for bioprocess medium
Related Categories
General description
HisPrep™ FF 16/10 columns are prepacked with Ni Sepharose™ 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose™ 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.
Features and Benefits
- Optimized for convenient scale-up purification of histidine-tagged proteins.
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
- Negligible leakage of Ni2+.
- High binding capacity, approx. 40 mg/mL medium.
- Convenient and time-saving 20 mL prepacked HiPrep™ format.
Storage and Stability
Analysis Note
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Certificates of Analysis (COA)
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Protocols
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