GE28-9365-50
GSTPrep™ Fast Flow 16/10
Cytiva 28-9365-50
About This Item
Recommended Products
ligand
glutathione and 10-carbon linker arm
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pkg of 20 mL
manufacturer/tradename
Cytiva 28-9365-50
parameter
<10 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
bed size
16 mm × 100 mm
bed volume
20 mL
column I.D.
16 mm
matrix
highly cross-linked 4% agarose
average diameter
90 μm
cleaning
3-12
working range
3-12
capacity
>10 mg/mL binding capacity(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
Related Categories
General description
Application
Features and Benefits
- Fast and easy one-step, scale-up purification of GST-tagged Proteins.
- Binds approx. 200 mg recombinant GST/column
- Mild elution conditions preserving Protein antigenicity and function.
- Simple operation with a pump or chromatography system, such as ÄKTA® design.
Storage and Stability
Analysis Note
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.
This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.
This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.
Protocols
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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