GE17-0965-05
Phenyl Sepharose™ 6 Fast Flow / Low Sub
Cytiva 17-0965-05, pack of 200 mL
About This Item
Recommended Products
ligand
phenyl
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 200 mL
manufacturer/tradename
Cytiva 17-0965-05
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
2-14
working range
3-13
suitability
suitable for bioprocess medium
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General description
Application
Phenyl Sepharose™ 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages. Two levels of ligand substitution degree help to find the optimal selectivity and binding capacity for a given application.
Phenyl Sepharose™ 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Phenyl Sepharose™ 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Standard aromatic HIC media based on Sepharose™ Fast FLow base matrix derivatized via uncharged, chemically-stable ether linkages.
- Designed for initial and intermediate purification requiring Low to medium hydrophobicity
- Two levels of substitution help find optimal selectivity and binding capacity for a given application
- BioProcess medium supported for industrial applications and well-established in approved processes
- The hydrophilic nature of the base matrix ensures Low levels of non-specific binding.
Storage and Stability
Analysis Note
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
The correct choice of salt and salt concentration are the most important parameters that influence capacity and final selectivity. The objective is to optimize conditions to achieve the required selectivity to bind the target protein(s) and ensure that the majority of impurities pass through the column.
Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with Cytiva media are detailed, including sample application, load volume and temperature
Protocols
Selectivity of HIC medium from Cytiva is based on properties such as hydrophobicity, porosity and particle size.
Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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