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GE28-9909-44

Sigma-Aldrich

Superdex® 200 Increase 10/300 GL

Cytiva 28-9909-44, column L × I.D. 30 cm × 10 mm, 8.6 μm particle size

Synonym(s):

Superdex Increase

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

glass column
polyethylene filter

product line

Superdex®

mol wt

10000-600000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9909-44

parameter

<1.8 mL/min flow rate
<1.80 mL/min flow rate
25-500 μL sample volume
3.0 Mpa (580 psi)

bed volume

~24 mL

column L × I.D.

30 cm × 10 mm

matrix

agarose-dextran composite
polymeric

particle size

8.6 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

Related Categories

General description

Superdex Increase 200 is a column for high resolution gel filtration in small scale (mg) preparative purification and also for characterization and analysis of proteins with molecular weights between 10,000 and 600,000, such as aggregate analysis of antibodies, size-homegeneity analysis of membrane proteins, or protein-protein interactions. Pre-packed with small rigid agarose beads for high flow rates and pH stability.

Suitability

For both preparative and analytical purposes
Gel filtration for small-scale (25-500 mL) preparative purification.
Long term use: all commonly used aqueous buffers, up to 8 M urea, ionic and non-ionic detergents, up to 6M guanidine hydrochloride

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

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Legal Information

Superdex is a registered trademark of Cytiva

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

This page shows how to perform a purification of His-tagged membrane proteins.

Protocols

Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

This page shows how to refold proteins using gel filtration and products from Cytiva.

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

This page discusses how to do a purification with SOURCE™ media from Cytiva.

Related Content

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service