MG-63 Cell Line human
86051601, human bone, Fibroblast
Synonym(s):
MG63 Cells
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About This Item
UNSPSC Code:
41106514
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product name
MG-63 Cell Line human, 86051601
biological source
human bone
description
Human osteosarcoma
growth mode
Adherent
karyotype
Modal no. 66; hypotriploid
morphology
Fibroblast
products
Inducible interferon
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human osteosarcoma
Cell Line Description
From an osteosarcoma of a 14 year old male. Produces high yields of interferon following superinduction with polyinosinic acid-polycytidilic acid, cyclohexamide and actinomycin. Antigenically, MG-63 interferon is more closely related to human fibroblast than to leukocyte interferon.
Application
Human MG-63 cell line has been used:
- to investigate the cytotoxicity of acrylic bone cement extracts on MG-63 cells
- to fabricate three-dimensional (3-D) niches and study the cell viability, adhesion and proliferation of MG-63 cells
- as a positive control for immunostaining procedure
- to study the interactions and effects of lysophosphatidate functionalized titanium on MG-63 cells
Interferon-induction studies
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 9.3
TPOX: 8,11
vWA: 16,19
CSF1PO: 10,12
D13S317: 11
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 9.3
TPOX: 8,11
vWA: 16,19
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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