RNASEA-RO
Roche
RNase A
from bovine pancreas
Synonym(s):
Rnase
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About This Item
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biological source
bovine pancreas
Quality Level
form
dry powder
specific activity
50 U/mg (+25°C, RNA as substrate)
mol wt
Mr = 13,700
packaging
pkg of 100 mg (10109169001)
pkg of 25 mg (10109142001)
manufacturer/tradename
Roche
parameter
65 °C optimum reaction temp.
technique(s)
flow cytometry: suitable
optimum pH
7.0-7.5
storage temp.
2-8°C
Related Categories
General description
Bovine pancreatic RNase A is a member of the RNase A protein superfamily. It is one of the most characterized proteins, and is a kidney shaped basic protein. This protein is composed of 124 amino acids. In its native form, RNase A exists as a homodimer.
Application
- For analytical purposes
- Isolation of DNA (for this purpose, RNase A should be boiled)
- For cell cycle analysis by flow cytometry and propidium iodide (PI) staining
Biochem/physiol Actions
RNase A catalyzes the transphosphorylation and degradation of RNA. It was the first protein identified to exhibit DNA melting functionality. This enzyme binds to several binding sites on single stranded (ss) RNA polynucleotide chain before degrading it. It interacts with ssDNA in a similar manner, which is responsible for its ‘DNA unwinding′ property. RNase A dimer obtained through tandemization shows toxicity to cancer cells.
Unit Definition
One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under assay conditions. A0 to A1 corresponds to the total conversion, A1 being the final absorbance.
Reconstitution
RNase A can be dissolved at a concentration of 1 to 10 mg/ml in 10 mM Tris-HCl, pH 7.5, 15 mM NaCl, heated to 100 °C for 15 minutes to inactivate contaminating DNases and cooled slowly to room temperature and dispend into aliquots. Roche recommends subsequent storage at -15 to -25 °C.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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