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AB1924

Sigma-Aldrich

Anti-Integrin α4 Antibody, cytoplasmic on CT

serum, Chemicon®

Synonym(s):

CD49d

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse, chicken, hamster, rat, pig

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable

NCBI accession no.

NM_000885.4

UniProt accession no.

P13612

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

chicken ... Itga4(424121)
human ... ITGA4(3676)
mouse ... Itga4(16401)
pig ... Itga4(100521477)
rat ... Itga4(311144)

Specificity

Integrin alpha 4. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine-labeled lymphoid cells. No cross-reactivity to alpha 1, alpha 2, alpha 3, alpha 5, alpha 6 or alpha V. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and it is thus intracellular.

Immunogen

Epitope: cytoplasmic domain, C-terminus
Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human alpha 4 integrin subunit.

Application

Anti-Integrin α4 Antibody, cytoplasmic on C-terminus is an antibody against Integrin α4 for use in ELISA, IP, RIA, WB, IH.
ELISA/RIA: 1:1000.

Western blot: 1:1000, non-reduced conditions; blocking should be with 1-3% BSA. Recognizes a 140 kDa human alpha 4 band under non-reducing conditions.

Immunohistochemistry: 1:500

Immunoprecipitation: 5 μL of antibody is sufficient to precipitate alpha4 from 5x106 cells.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins

Linkage

Replaces: 04-1131

Physical form

Liquid rabbit antiserum with 0.05% sodium azide.

Storage and Stability

Maintain at -20ºC in undiluted aliquots for up to 12 months upon date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Regulation of human osteoblast integrin expression by orthopedic implant materials.
R K Sinha, R S Tuan, R K Sinha, R S Tuan, R K Sinha, R S Tuan
Bone null
J Linfor et al.
Journal of reproduction and fertility, 120(1), 65-72 (2000-09-28)
Integrin molecules are cell adhesion molecules that are thought to be involved in sperm-oocyte interaction in rodents and humans. The objective of this study was to evaluate whether integrin molecules were present on the surface of pig oocytes, consistent with
Regulation of integrin function by CD47 ligands. Differential effects on alpha vbeta 3 and alpha 4beta1 integrin-mediated adhesion.
Barazi, HO; Li, Z; Cashel, JA; Krutzsch, HC; Annis, DS; Mosher, DF; Roberts, DD
The Journal of Biological Chemistry null
Expression of alphav, alpha4, alpha5 and beta3 integrin subunits, fibronectin and vitronectin in goat peri-implantation
Garcia, P. et al.
Animal Reproduction Science, 80(1-2), 91-100 (2004)
Integrins mediate adherence and migration of T lymphocytes on human peritoneal mesothelial cells.
Wang, HH; Lee, TY; Lin, CY
Kidney International null
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