203118
Biotin-NHS, Water-Soluble
Synonym(s):
Biotin-NHS, Water-Soluble, Sulfobiotin-NHS, Sulfosuccinimidobiotin
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About This Item
Empirical Formula (Hill Notation):
C14H18N3O8S2 · Na
Molecular Weight:
443.43
UNSPSC Code:
41116133
NACRES:
NA.41
Recommended Products
form
solid
Quality Level
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated
color
off-white
solubility
DMSO: 10 mg/mL
distilled water: 4 mg/mL
shipped in
ambient
storage temp.
2-8°C
General description
Reacts with primary amines to biotinylate proteins under mild conditions. Since it is water-soluble, this compound can be used to biotinylate cell-surface proteins and proteins that are sensitive to organic solvents.
Water-soluble form of biotin-NHS. Water solubility is extremely convenient, eliminating the need to dissolve the reagent in an organic solvent such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) prior to use.
Warning
Toxicity: Standard Handling (A)
Reconstitution
Unstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Analysis Note
homogeneous single spot by TLC
Other Notes
Lisanti, M.P., et al. 1989. J. Cell Biol.109, 2145.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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