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QBD10200

Sigma-Aldrich

NHS-dPEG®4-biotin

Synonym(s):

(+)-Biotin-PEO4-NHS-propionate, NHS-PEO4-Biotin, Polyethylene glycol

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About This Item

Empirical Formula (Hill Notation):
C25H40N4O10S
CAS Number:
459426-22-3
Molecular Weight:
588.67
UNSPSC Code:
12161502
PubChem Substance ID:
329823890
NACRES:
NA.22

assay

>90%

form

solid or viscous liquid

reaction suitability

reagent type: cross-linking reagent
reaction type: Biotinylations
reactivity: amine reactive
reagent type: linker

polymer architecture

shape: linear
functionality: monofunctional

shipped in

ambient

storage temp.

−20°C

SMILES string

O=C(ON1C(CCC1=O)=O)CCOCCOCCOCCOCCNC(CCCCC2C3NC(NC3CS2)=O)=O

InChI

1S/C25H40N4O10S/c30-20(4-2-1-3-19-24-18(17-40-19)27-25(34)28-24)26-8-10-36-12-14-38-16-15-37-13-11-35-9-7-23(33)39-29-21(31)5-6-22(29)32/h18-19,24H,1-17H2,(H,26,30)(H2,27,28,34)/t18-,19-,24-/m0/s1

InChI key

DTLVBHCSSNJCMJ-JXQFQVJHSA-N

Related Categories

General description

NHS-dPEG®4-biotin is a water-soluble cross-linking reagent used in biotinylation reactions. The N-hydroxysuccinimidyl (NHS) moiety of NHS-dPEG®4-biotin forms a stable amide bond with free primary amines, such as the amines on the side chain of lysine. The biotin moiety is used in a variety of applications, including labeling, supramolecular building, affinity chromatography, pull-down assays, and the production of biotinylated antibodies.

Features and Benefits

NHS-dPEG®4-biotin is used extensively to label molecules that contain a free amine with biotin. The single molecular weight NHS-dPEG®4-biotin has a long spacer of precise length (19.2 Å) between the biotin moiety and the labeled molecule. This dPEG® spacer imparts a high degree of water solubility to the biotin label. For biotinylated peptides and proteins, the dPEG® spacer dramatically reduces aggregation and precipitation of the modified molecule and reduces non-specific binding of the molecule, both of which are common problems with molecules that have been biotinylated with the widely used NHS-LC-biotin.

Legal Information

Products Protected under U.S. Patents # 7,888,536 B2
dPEG is a registered trademark of Quanta BioDesign

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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