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L9043

Sigma-Aldrich

Lysostaphin from Staphylococcus simulans

recombinant, expressed in E. coli, lyophilized powder

Synonym(s):

Glycyl-glycine Endopeptidase

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About This Item

CAS Number:
9011-93-2
Enzyme Commission number:
3.4.24.75 (BRENDA, IUBMB)
MDL number:
MFCD00131556
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bacterial (Streptomyces globisporus)

Quality Level

200

recombinant

expressed in E. coli

Assay

≥90% (HPLC)

form

lyophilized powder

specific activity

≥3000 unit/mg solid

technique(s)

molecular cloning: suitable

suitability

suitable for sequence analysis test

application(s)

life science and biopharma

shipped in

dry ice

storage temp.

−20°C

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General description

Lysostaphin, a zinc metalloenzyme, is mainly secreted as a proenzyme. It acts as an anti-staphylococcal agent and is highly stable at pH 4 and 5°C.

Application

Lysostaphin from Staphylococcus simulans has been used in a study to assess molecular cloning, sequencing, and expression of lytM, a unique autolytic gene of Staphylococcus aureus. It has also been used in a study to investigate the sequence analysis of a Staphylococcus aureus gene encoding a peptidoglycan hydrolase activity.

Biochem/physiol Actions

A protein complex with highly specific lytic activity against Staphylococcus species, including Staphylococcus aureus.
Lysostaphin possesses a lytic action against Staphylococcus aureus, which is used to treat antibiotic-resistant staphylococcal infections. It has the properties of enzymes, such as glycylglycine endopeptidase, endo-β-N-acetyl glucosamidase and N-acetyl muramyl-L-alanine amidase. Lysostaphin is also used as a preservative in food industry and in clinical labs for rapid screening.
The antimicrobial activity of lysostaphin, a metalloendopeptidase expressed by Staphylococcus simulans, is enhanced by binding to the C′ terminus of the membrane-associated Trap protein of Staphylococcus aureus.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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C-terminus of TRAP in Staphylococcus can enhance the activity of lysozyme and lysostaphin.
Yang, G.
Acta Biochimica et Biophysica Hungarica, 40, 452-458 (2008)
L Ramadurai et al.
Journal of bacteriology, 179(11), 3625-3631 (1997-06-01)
A gene encoding an autolytic activity was identified in an autolysis-deficient mutant (Lyt-) of Staphylococcus aureus which produces only a single band in autolytic-activity gels (N. Mani, P. Tobin, and R. K. Jayaswal, J. Bacteriol. 175:1493-1499, 1993). An open reading
X Wang et al.
Gene, 102(1), 105-109 (1991-06-15)
The nucleotide (nt) sequence of a 2.0-kb NheI-XbaI DNA fragment containing a peptidoglycan hydrolase-encoding gene, lytA, tentatively identified as encoding an N-acetylmuramyl-L-alanine amidase, from Staphylococcus aureus, was determined. The nt sequencing revealed an open reading frame (ORF) of 1443 bp
Lysostaphin: an antistaphylococcal agent
Kumar J K
Applied Microbiology and Biotechnology, 80(4), 555-561 (2008)
Grégory Francius et al.
Journal of bacteriology, 190(24), 7904-7909 (2008-10-07)
The advent of Staphylococcus aureus strains that are resistant to virtually all antibiotics has increased the need for new antistaphylococcal agents. An example of such a potential therapeutic is lysostaphin, an enzyme that specifically cleaves the S. aureus peptidoglycan, thereby
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