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L7386

Sigma-Aldrich

Lysostaphin from Staphylococcus staphylolyticus

lyophilized powder, Protein 50-70 % by biuret, ≥500 units/mg protein

Synonym(s):

Glycyl-glycine Endopeptidase

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About This Item

CAS Number:
9011-93-2
Enzyme Commission number:
3.4.24.75 (BRENDA, IUBMB)
MDL number:
MFCD00131556
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

microbial (Staphylococcus staphylotyticus)

Quality Level

300

form

lyophilized powder

specific activity

≥500 units/mg protein

mol wt

25 kDa

composition

Protein, 50-70% biuret

packaging

pkg of (sold on basis of mg protein)

solubility

water: 2.00-2.10 mg/mL, clear to slightly hazy, colorless to light yellow

antibiotic activity spectrum

Gram-positive bacteria

application(s)

diagnostic assay manufacturing
diagnostic assay manufacturing

Mode of action

cell wall synthesis | interferes

shipped in

wet ice

storage temp.

−20°C

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General description

Chemical structure: peptide

Biochem/physiol Actions

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic.
pH Optimum for activity: ~7.5

Unit Definition

One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lisbeth Drozd Lund et al.
PloS one, 11(2), e0149092-e0149092 (2016-02-13)
Staphylococcus aureus is a major human pathogen that has evolved very efficient immune evading strategies leading to persistent colonization. During different stages of growth, S. aureus express various surface molecules, which may affect the immune stimulating properties, but very little
Ian R Monk et al.
Nature communications, 10(1), 3067-3067 (2019-07-13)
WalKR (YycFG) is the only essential two-component regulator in the human pathogen Staphylococcus aureus. WalKR regulates peptidoglycan synthesis, but this function alone does not explain its essentiality. Here, to further understand WalKR function, we investigate a suppressor mutant that arose
Judy J J Ou et al.
Frontiers in cellular and infection microbiology, 6, 187-187 (2017-01-14)
Background:Staphylococcus aureus (S. aureus) small colony variants (SCVs) can survive within the host intracellular milieu and are associated with chronic relapsing infections. However, it is unknown whether host invasion rates and immune responses differ between SCVs and their wild-type counterparts.
Catherine Bennett et al.
International forum of allergy & rhinology, 9(8), 918-925 (2019-06-05)
Chronic rhinosinusitis (CRS) is a common disease, often refractory to conventional antimicrobial treatment. In this study we investigate the antimicrobial and anti-inflammatory effects of adding kappa-carrageenan to a commercially available sinus rinse. Kappa-carrageenan was added to Flo CRS and Flo
Igor Belyansky et al.
The Journal of surgical research, 171(2), 409-415 (2011-06-24)
Lysostaphin (LS), a naturally occurring Staphylococcal endopeptidase, has the ability to penetrate biofilm, and has been identified as a potential antimicrobial to prevent mesh infection. The goals of this study were to determine if LS adhered to porcine mesh (PM)
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