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L5542

Sigma-Aldrich

LB Agar Plates

pre-poured LB agar plates for E. coli

Synonym(s):

pre-poured LB agar plates

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About This Item

UNSPSC Code:
12352200

grade

for molecular biology

Quality Level

sterility

sterile; autoclaved

form

plates (10 cm)
plates (10cm, 20-25ml each)

capacity

20-25 mL

storage temp.

2-8°C

suitability

nonselective for Escherichia coli
nonselective for coliforms

General description

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of Escherichia coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
LB Agar Plates has been used:
  • to culture bacteria like, Escherichia coli strain INV- α and methicillin-susceptible Staphylococcus aureus
  • to culture numerous bacteria and is used in strain storage method
  • to analyse the egestion of midgut microbes during sand fly bites

Features and Benefits

Lennox LB Agar plates provide:
  • Convenient, ready-to-use 10cm format
  • Standard formulation

Components

15g/L Agar
10g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ
Kuru E, et al.
Nature Protocols, 10(1), 33-33 (2015)
Bacteria Detection and Differentiation Using Impedance Flow Cytometry
Clausen C, et al.
Sensors, 18(10), 3496-3496 (2018)
Gut microbes egested during bites of infected sand flies augment severity of leishmaniasis via inflammasome-derived IL-1beta
Dey R, et al.
Cell host & microbe, 23(1), 134-143 (2018)
Alex Golberg et al.
AAPS PharmSciTech, 10(3), 881-886 (2009-07-03)
The purpose of this study was to examine the feasibility of using irreversible electroporation (IRE) as a non-chemical method for eliminating microorganisms of liquid drugs. The studied drug was a topical ophthalmic medication, a pharmaceutical field in which the problem
Casper Hyttel Clausen et al.
Sensors (Basel, Switzerland), 18(10) (2018-10-20)
Monitoring of bacteria concentrations is of great importance in drinking water management. Continuous real-time monitoring enables better microbiological control of the water and helps prevent contaminated water from reaching the households. We have developed a microfluidic sensor with the potential

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