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L0168

Sigma-Aldrich

LB Agar Ampicillin - 50, Plates

pre-poured LB agar plates with 50 μg/ml ampicillin

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About This Item

UNSPSC Code:
12352200

grade

for molecular biology

Quality Level

100

sterility

sterile; autoclaved

form

plates (10 cm)
plates (10cm, 20-25ml each)

availability

available only in USA

capacity

20-25 mL

storage temp.

2-8°C

suitability

selective for Escherichia coli
selective for coliforms

Related Categories

General description

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for selective cultivation of E. coli strains that contain plasmids conferring ampicillin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins.

Features and Benefits

Miller′s LB Agar plates provide:
  • Convenient, ready-to-use 10cm format
  • Standard formulation plus antibiotic

Components

15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml ampicillin

Other Notes

The plates must be used within two months of the manufacture date to ensure the antibiotics are fully active. The product may be shipped up until its expiration date but is generally shipped 2-4 weeks before expiration.

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Protein Expression Systems

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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