Recommended Products
biological source
mouse blood
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Mouse Myeloma
Cell Line Description
P3x63Ag8U.1 is a derivative of the P3x63Ag8 cell line (Sigma Catalogue number. 85011401) from the mouse myeloma MOPC21, which was derived from a female BALB/c mouse. This variant no longer synthesises gamma-1 chains but still produces kappa light chains which are not secreted.
Application
This cell line is useful for fusion to spleen cells to produce hybridomas or for hybridisation studies with other myelomas or lymphomas. Can also be used for immunoglobulin production.
Culture Medium
DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures at 5-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
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Articles
Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines at sigmaaldrich.com
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