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HF2x653

90012609

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About This Item

UNSPSC Code:
41106514

biological source

Human x Mouse Hybridoma hybrid

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human (WI-L2-729-FF2) x mouse (P3X63/Ag8.653) heterohybridoma

Cell Line Description

Generated by electro-acoustic fusion of 2 lines. Parent: WI-L2-729-HF2. Murine myeloma: P3X63/Ag8.653. Non-immunoglobulin secreting, HAT-sensitive, resistant to ouabain at greater than 100uM, will fuse with normal human lymphocytes and lymphoblastoids.

Application

Fusion partner

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Hybridoma Fusion Partners Cell Lines

Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines at sigmaaldrich.com

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