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Sigma-Aldrich

Leukocyte Alkaline Phosphatase Kit

based on naphthol AS-BI and fast blue BB salt

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About This Item

UNSPSC Code:
12352200
eCl@ss:
42010105
NACRES:
NA.47

Quality Level

500

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

application(s)

hematology
histology

shipped in

wet ice

storage temp.

2-8°C

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Application

Leukocyte Alkaline Phosphatase (LAP) kits are intended for the qualitative demonstration of alkaline phosphatase activity in white blood cells.
Peripheral blood or bone marrow preparations are fixed to a microscope slide. The film is then incubated in a mixture of naphthol AS-BI alkaline solution with fast blue BB. The resulting insoluble diffuse, blue dye deposit indicates sites of alkaline phosphatase activity.

Kit Components Only

Product No.
Description
  • Citrate Solution (915) 50 mL
  • FBB-Alkaline Solution (863) 10 mL
  • Naphthol AS-BI Alkaline Solution (861) 10 mL
  • Neutral Red Solution (N6264) 50 mL
  • Sodium Nitrite Solution (914) 10 mL

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Supplementary Hazards

EUH071

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Certificates of Analysis (COA)

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Barbara Klotz et al.
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Gregory M Cooper et al.
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Sandra Bermeo et al.
Journal of cellular biochemistry, 118(12), 4425-4435 (2017-04-28)
Mutations in MAN1, a protein of the nuclear envelope, cause bone phenotypes characterized by hyperostosis. The mechanism of this pro-osteogenic phenotype remains unknown. We increased and decreased MAN1 expression in mesenchymal stem cells (MSC) upon which standard osteogenic and adipogenic
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