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52062

Millipore

LB-Agar, high salt

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Luria Bertani Agar, high salt

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About This Item

UNSPSC Code:
41171605
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
casein enzymic hydrolysate, 10 g/L
sodium chloride, 10 g/L
yeast extract, 5 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.5±0.2 (25 °C)

application(s)

clinical testing
food and beverages
life science and biopharma
microbiology

suitability

nonselective for Escherichia coli
nonselective for bacteria (General Media)
nonselective for coliforms

Related Categories

General description

LB-Agar, high salt is a growth media used in microbiology. It supports Escherichia coli growth to an optical density at 600 nm (OD600) of 7.

Application

LB-Agar, high salt may be used as a growth media in the determination of phylogenetic groups (A, B1, B2, and D) of Escherichia coli bacteria using triplex PCR technique.
Luria Media are used for the cultivation and maintenance of recombinant strains of E. coli for genetic and molecular studies.

Preparation Note

Suspend 40 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Dispense as desired.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCD8141
BCCB7070
BCBS9324V
BCBR4424V
BCBP3565V

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Escherichia coli Physiology in Luria-Bertani Broth.
Sezonov G, et al.
Journal of Bacteriology, 189(23), 8746?8749-8746?8749 (2007)
Rapid and simple determination of theEscherichia coli phylogenetic group.
Clermont O, et al.
Applied and Environmental Microbiology, 66(10), 4555-4558 (2000)
Commercial Lysogeny Broth culture media and oxidative stress: a cautious tale.
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