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XGAL-RO

Roche

X-Gal

solubility: in DMF, soluble, solubility: in methanol, soluble, storage temp.:2-8°C

Synonym(s):

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside, bromo-4-chloro-3-indolyl-β-d-galactopyranoside, 5-, 5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal

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About This Item

Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
7240-90-6
Molecular Weight:
408.63
Beilstein:
1402009
MDL number:
MFCD00005666
UNSPSC Code:
12352204
PubChem Substance ID:
329830810
NACRES:
NA.28

description

5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside

Quality Level

100

Assay

99%

form

solid

mol wt

408.63 g/mol

packaging

pkg of 1 g (10745740001)
pkg of 100 mg (03117073001)
pkg of 100 mg (11680293001)
pkg of 2.5 g (10703729001)
pkg of 250 mg (10651745001)

manufacturer/tradename

Roche

concentration

20 mg/mL

technique(s)

activity assay: suitable

color

white

solubility

DMF: soluble
methanol: soluble

application(s)

genomic analysis
life science and biopharma

storage temp.

2-8°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI key

OPIFSICVWOWJMJ-AEOCFKNESA-N

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General description

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. It is soluble in dimethylformamide and methanol. The product is not soluble in water.

Application

X-Gal has been used for immunohistocytochemistry applications. It is also the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.
X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

Quality

Chromatographically homogeneous

Physical form

Crystals, white powder

Preparation Note

Working solution: Stock solution:
  • 20 mg/ml in dimethyl formamide (DMF), clear, colorless solution
  • A590 nm: 0.01

Storage conditions (working solution): X-gal in solution (i.e., dissolved in DMF 2% (w/v)) is stable for several weeks when stored in the dark at 2 to 8 °C; when the solution is stored at -15 to -25 °C, it is stable for one year.

Other Notes

For life science research only. Not for use in diagnostic procedures.

related product

Product No.
Description
Pricing

94433

β-Galactosidase stain

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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5-Bromo-4-chloro-3-indolyl α-D-galactopyranoside ≥98.0% (HPLC)

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A I Geller et al.
Science (New York, N.Y.), 241(4873), 1667-1669 (1988-09-23)
A defective herpes simplex virus 1 (HSV-1) vector, pHSVlac, has been developed that contains a transcription unit that places the Escherichia coli lacZ gene under the control of the HSV-1 immediate early 4/5 promoter. The vector pHSVlac was propagated with
Maryvonne Arnaud et al.
Applied and environmental microbiology, 70(11), 6887-6891 (2004-11-06)
A shuttle vector designated pMAD was constructed for quickly generating gene inactivation mutants in naturally nontransformable gram-positive bacteria. This vector allows, on X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) plates, a quick colorimetric blue-white discrimination of bacteria which have lost the plasmid, greatly facilitating clone
A R Yallowitz et al.
Cell death and differentiation, 21(4), 645-654 (2014-01-21)
In embryogenesis, p63 is essential to develop mammary glands. In the adult mammary gland, p63 is highly expressed in the basal cell layer that comprises myoepithelial and interspersed stem/progenitor cells, and has limited expression in luminal epithelial cells. In adult

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