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P5459

Sigma-Aldrich

Protease aus Bacillus licheniformis

glycerol solution (50%)

Synonym(e):

Alkalase, Proteinase aus Bacillus licheniformis, Subtilisin A, Subtilisin Carslberg

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About This Item

CAS-Nummer:
9014-01-1
EC-Nummer:
3.4.21.62 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00132092
UNSPSC-Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

Biologische Quelle

Bacillus licheniformis

Qualitätsniveau

200

Form

glycerol solution (50%)

Spezifische Aktivität

≥7 units/mg protein (biuret)

Konzentration

≥50 mg/mL

Löslichkeit

0.85% sodium chloride: soluble, clear (Diluted)
H2O: miscible

Lagertemp.

2-8°C

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Verwandte Kategorien

Allgemeine Beschreibung

The enzyme hydrolyzes gelatin optimally at 50 °C over the range of pH 8-10. It is stable between pH 6-10 at 50 °C. Commercially, it is used in laundry detergent formulations and also for the recovery of silver from photographic emulsions.

Anwendung

Protease from Bacillus licheniformis has been used to digest the neurolemma in the antennal lobe of the moth; Heliothis virescens.
Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations. Protease from Bacillus licheniformis, product P5459, has been used to hydrolyze muscle during the isolation of mitochondria from skeletal muscle.

Biochem./physiol. Wirkung

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.

Verpackung

Package size based on protein content.

Einheitendefinition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Piktogramme

CorrosionHealth hazard
GHS05,GHS08

Signalwort

Danger

Gefahreneinstufungen

Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Frontiers in cardiovascular medicine, 7, 23-23 (2020-03-21)
Radiation therapy is received by over half of all cancer patients. However, radiation doses may be constricted due to normal tissue side effects. In thoracic cancers, including breast and lung cancers, cardiac radiation is a major concern in treatment planning.
H T Skiri et al.
Chemical senses, 29(3), 253-267 (2004-03-30)
The primary olfactory centre, the antennal lobe of Heliothis virescens moths, contains 62 glomeruli which process plant odour information and four male-specific glomeruli which form the macroglomerular complex, involved in processing information about pheromone and interspecific signals. Using calcium imaging
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Protokolle

Enzymatic Assay of Protease Using Casein As a Substrate

To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate.

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