H8416
Stem Cell Factor human
SCF, recombinant, expressed in HEK 293 cells, suitable for cell culture
Synonym(e):
SCF, c-Kit ligand
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About This Item
Empfohlene Produkte
Biologische Quelle
human
Qualitätsniveau
Rekombinant
expressed in HEK 293 cells
Assay
≥95% (SDS-PAGE)
Form
lyophilized powder
Wirksamkeit
≤200 ng/mL ED50
Qualität
endotoxin tested
Mol-Gew.
dimer 35-45 kDa (glycosylated)
Verpackung
pkg of 5x10 μg
pkg of 10 μg
pkg of 100 μg
Lagerbedingungen
avoid repeated freeze/thaw cycles
Methode(n)
cell culture | mammalian: suitable
Verunreinigungen
≤1 EU/μg
UniProt-Hinterlegungsnummer
Lagertemp.
−20°C
Angaben zum Gen
human ... KITLG(4254)
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Allgemeine Beschreibung
The KITLG (KIT ligand) gene is mapped to human chromosome 12q21.32.
Biochem./physiol. Wirkung
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells.
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.
Stem cell factor (SCF) of granulosa cell origin, efficiently contributes to the follicular development and oocyte maturation. SCF controls tumor development and progression. Elevated SCF gene expression is observed in hepatocellular carcinoma.
Angaben zur Herstellung
SCF is expressed in human HEK 293 cells as a glycosylated monomer with an apparent molecular mass of 35-45 kDa due to glycosylation. Production in human 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
Hinweis zur Analyse
The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
Rechtliche Hinweise
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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The receptor tyrosine kinase, c-kit, and its ligand, stem cell factor (SCF), function in a diverse range of biological functions. The role of c-kit in the maintenance and survival of hematopoietic stem cells and of mast cells is well recognized.
Sibylle A Brenner et al.
Immunology, 143(2), 174-183 (2014-04-05)
Mast cells are now considered sentinels in immunity. Given their location underneath the gastrointestinal barrier, mast cells are entrusted with the task of tolerating commensal microorganisms and eliminating potential pathogens in the gut microbiota. The aim of our study was
Artikel
Read article on hematopoietic cytokines and hematopoiesis
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