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Merck

CELLMM2

Millipore

FLAG® M Purification Kit

For Mammalian expression systems.

Synonym(e):

Anti-ddddk, Anti-dykddddk

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About This Item

UNSPSC-Code:
12352200
NACRES:
NA.32

Qualitätsniveau

Methode(n)

protein extraction: suitable

Versandbedingung

wet ice

Lagertemp.

−20°C

Anwendung

The FLAG Purification Kit utilizes CelLytic Reagents for rapid and efficient cell lysis and protein extraction and ANTI-FLAG® M2 affinity gel for affinity purification of active FLAG fusion proteins.

Learn more product details in our FLAG® application portal.

Leistungsmerkmale und Vorteile

  • Includes ready to use reagents, columns, and a detailed protocol for affinity purification of FLAG fusion proteins.
  • ANTI-FLAG M2 Affinity Gel allows efficient binding of FLAG fusion proteins without the need for preliminary steps and calibrations.
  • Two alternatives for efficient elution conditions (by acidic conditions or by competition with FLAG peptide).

Verpackung

Sufficient for 3-5 uses of 1-ml affinity purification column.

Sonstige Hinweise

Kit contains CelLytic™ M for cell lysis and protein extraction from Mammalian cells.

Rechtliche Hinweise

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.
Beschreibung
SDB

  • C2978CelLytic M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.SDB

  • SAE0194Purified 3xFLAG® peptide, ≥95% (HPLC), lyophilized powderSDB

  • A2220ANTI-FLAG® M2 Affinity Gel, purified immunoglobulin, buffered aqueous glycerol solutionSDB

  • C2103Chromatography columns, general-purpose, volume 10 mL, Overall H 5 in.SDB

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Verwandter Inhalt

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

Protein expression technologies for expressing recombinant proteins in E. coli, insect, yeast, and mammalian expression systems for fundamental research and the support of therapeutics and vaccine production.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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