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Merck

CB0500

Sigma-Aldrich

CelLytic B Plus Kit

For bacterial lysis

Synonym(e):

Lysis and protein extraction kit

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

EG-Nummer:
UNSPSC-Code:
41106511
NACRES:
NA.56

Qualitätsniveau

Verwendung

 kit sufficient for 50 g fresh or frozen cell paste lysis.

Methode(n)

protein extraction: suitable

Versandbedingung

wet ice

Lagertemp.

−20°C

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Allgemeine Beschreibung

The CelLytic B Plus Kit is designed to efficiently lyse cells and extract proteins from both Gram negative, and difficult to lyse, Gram positive bacteria. This is accomplished using the standard CelLytic B, a proprietary non-ionic detergent in concert with lysozyme, Benzonase®, and protease inhibitors. This complete kit takes the guesswork out of protein extraction from a variety of bacterial species.

Anwendung

This kit is compatible with FLAG®, HIS-Select®, and glutathione S-transferase based affinity chroma­tography protein purification systems. The detergent included in the CelLytic B Lysis Reagent can be removed from the protein by dialysis or ammonium sulfate precipitation, if necessary. The final purity of the recombinant product is usually higher than that obtained from traditional extraction methods. This is because the crude protein solution containing the non-ionic detergent eliminates much of the non-specific binding that occurs during chromatography. The mild detergent does not interfere with many enzyme assays or protein assays.

Leistungsmerkmale und Vorteile

  • Lyse Gram positive and Gram negative bacteria
  • More efficient than sonication
  • Compatible with affinity purification
  • Isolate inclusion bodies for subsequent solubilization
  • Non-denaturing cell lysis preserves protein function

Sonstige Hinweise

The CelLytic B Plus Kit contains all of the reagents and chemicals necessary to lyse both Gram-negative and Gram-positive bacteria. This kit also includes protease inhibitors to help prevent the proteolytic breakdown of proteins.

Rechtliche Hinweise

Benzonase® Nuclease is manufactured by Merck KGgA, Darmstadt, Germany and its affiliates. Nycomed Pharma A/S (Denmark) claims worldwide patent rights to Benzonase Nuclease, which are licensed exclusively to Merck KGaA, Darmstadt, Germany.
Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.
Beschreibung
SDB

  • B7435CelLytic B Cell Lysis Reagent, For bacterial cell lysis, standard strengthSDB

  • E1014Benzonase® Nuclease, ≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solutionSDB

  • P8849Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solutionSDB

  • L3790Lysozyme from chicken egg white, 10 mg/mLSDB

Piktogramme

Health hazard

Signalwort

Danger

Gefahreneinstufungen

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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