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B4554

Sigma-Aldrich

BES

BioPerformance Certified, suitable for cell culture, ≥99.0%

Synonym(e):

N,N-Bis-(2-hydroxyethyl)-2-amino-ethansulfonsäure, N,N-Bis-(2-hydroxyethyl)-taurin

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About This Item

Empirische Formel (Hill-System):
C6H15NO5S
CAS-Nummer:
10191-18-1
Molekulargewicht:
213.25
Beilstein:
1781572
EG-Nummer:
233-465-5
MDL-Nummer:
MFCD00007533
UNSPSC-Code:
12161700
PubChem Substanz-ID:
24891788
NACRES:
NA.25

Qualität

BioPerformance Certified

Assay

≥99.0%

Form

crystalline

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

endotoxin and bioburden, tested

pH-Wert

2.5-5.0 (25 °C, 213.3 g/L)

Nützlicher pH-Bereich

6.4-7.8

pKa (25 °C)

7.1

Löslichkeit

water: 0.5 g/mL, clear, colorless

Anwendung(en)

diagnostic assay manufacturing

Fremdaktivität

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

Lagertemp.

room temp

SMILES String

OCCN(CCO)CCS(O)(=O)=O

InChI

1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)

InChIKey

AJTVSSFTXWNIRG-UHFFFAOYSA-N

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Allgemeine Beschreibung

N-N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES) is a zwitterionic sodium sulphate buffer used in biochemistry and molecular biology research. BES buffer used as a secondary pH standard can help maintain a stable pH range in solutions, particularly in the range of 6.2-7.6 pH range of 6.6 to 7.4. It ​is used in a study to evaluate that buffers minimize the action of interfering factors in the bacterial endotoxins test. The application of BES as a buffer solution for calcium phosphate-mediated transfection of eukaryotic cells with plasmid DNA has also been studied.

Anwendung

BES buffer has been used:

  • as a supplement in opti-minimal essential medium (MEM) culture media to culture thymocytes for In vitro analysis of thymocyte apoptosis
  • as a component of borate buffer saline (BBS) for adeno-associated virus (AAV) vector production, extraction, and purification
  • as a component of diluent one for semen collection and cryopreservation

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Gloves

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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MES -Lösung suitable for, BioPerformance Certified, 1 M, suitable for cell culture

Sigma-Aldrich

M1317

MES -Lösung

TES ≥99% (titration)

Sigma-Aldrich

T1375

TES

Kohlensäure-Bicarbonat-Puffer capsule

Sigma-Aldrich

C3041

Kohlensäure-Bicarbonat-Puffer

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