Monoclonal Anti-FITC−Biotin antibody produced in mouse

Monoclonal Anti-FITC (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC).

FITC-BSA conjugate

Monoclonal Anti-FITC-Biotin antibody produced in mouse has been used:

• for amplification of signal in immunofluorescence assays
• in Ag-activated beads for flow-based micro immunoassay of parathyroid hormone and IL-5
• enzyme linked immunosorbent assay (ELISA)

Fluorochrome labeling provides a rapid and accurate localization of the site of antigen-antibody interaction when one of the reactants from parts of a cell, tissue or other biological structure. Fluorescein isothiocyanate (FITC) is a commonly used marker for antibodies in immunofluorescence techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labelled substances. FITC is widely used as a hapten to label different proteins and anti-FITC antibodies are used to identify these labeled proteins. Antibodies to FITC serve as universal indicator reagents by bridging FITC with another immunohistochemical reagent, such as alkaline phosphatase, horseradish peroxidase or biotin.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide

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