A9316
Anti-Maus-IgG (Gesamtmolekül)−Alkalische Phosphatase in Ziege hergestellte Antikörper
affinity isolated antibody, buffered aqueous glycerol solution
Synonym(e):
Goat Anti-Mouse IgG (whole molecule)−AP
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About This Item
Empfohlene Produkte
Biologische Quelle
goat
Qualitätsniveau
Konjugat
alkaline phosphatase conjugate
Antikörperform
affinity isolated antibody
Antikörper-Produkttyp
secondary antibodies
Klon
polyclonal
Form
buffered aqueous glycerol solution
Methode(n)
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000
Versandbedingung
wet ice
Lagertemp.
2-8°C
Posttranslationale Modifikation Target
unmodified
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Allgemeine Beschreibung
Goat polyclonal anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody is reactive with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM. Cross reactivity of the antibody conjugate is determined by ELISA. The conjugate shows no reactivity with rat IgG or rat serum proteins.
Immunoglobulins (Igs) belong to the immunoglobulin super-family. IgG is an abundant immunoglobulin present in human serum. The four classes of IgG are IgG1, IgG2, IgG3, and IgG4.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Anwendung
Surfactant Protein A wurde in bronchoalveolärer Flüssigkeit unter Verwendung von an alkalischer Phosphatase konjugiertem Anti-Maus-IgG von der Ziege als Sekundärantikörper bei μg/ml in TBS/Tween mit einer Endkonzentration von 0,5 M NaCl nachgewiesen.
Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in goat has been used as a secondary antibody in:
- histological studies in heart and liver grafts
- immunohistochemical studies of gangliosides and immunostaining of lymphoid organs
- western blot detection in blood samples
The level of antibodies specific to P. aeuginosa present in immunized mouse sera was determined by ELISA using alkaline phosphatase-conjugated goat anti-mouse IgG as the secondary antibody at a dilution of 1:1000.
Biochem./physiol. Wirkung
IgG1 deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection.
Sonstige Hinweise
Antibody adsorbed with rat serum proteins.
Physikalische Form
Solution in 0.05 M Tris, pH 8.0, containing 10 mM glycine, 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide.
Angaben zur Herstellung
Adsorbed to reduce background with rat samples.
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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