A2179
Anti-Mouse IgG (Fab specific)–Alkaline Phosphatase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Synonym(e):
Goat Anti-Mouse IgG (Fab specific)–AP
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(4)
About This Item
MDL-Nummer:
UNSPSC-Code:
12352203
Empfohlene Produkte
![Tributyl[(methoxymethoxy)methyl]stannane AldrichCPR](/deepweb/assets/sigmaaldrich/product/structures/405/394/e2659ee0-7927-4ef9-b0bc-dc331065f409/640/e2659ee0-7927-4ef9-b0bc-dc331065f409.png)
Biologische Quelle
goat
Qualitätsniveau
Konjugat
alkaline phosphatase conjugate
Antikörperform
affinity isolated antibody
Antikörper-Produkttyp
secondary antibodies
Klon
polyclonal
Form
buffered aqueous solution
Speziesreaktivität
mouse
Darf nicht reagieren mit
horse, human, bovine
Methode(n)
direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25
western blot (chemiluminescent): 1:80,000
Versandbedingung
wet ice
Lagertemp.
2-8°C
Posttranslationale Modifikation Target
unmodified
Suchen Sie nach ähnlichen Produkten? Aufrufen Leitfaden zum Produktvergleich
Verwandte Kategorien
Allgemeine Beschreibung
Immunoglobulins (Igs) belongs to the immunoglobulin super-family. Each immunoglobin have two heavy (H) and two light (L) chains held together by disulfide linkages.
Immunogen
Purified mouse IgG, Fab fragment
Anwendung
Alkaline phosphatase-conjugated goat anti-mouse IgG (Fab specific) was used as the secondary antibody in ELISA assays to measure the reacitivity of mABs to thyrogobulin peptides. Absorbance was measured at 405 nm.
Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in goat has been used in:
- western blotting
- immunoblotting
- enzyme linked immunosorbent assay (ELISA)
- immunohistochemistry
Biochem./physiol. Wirkung
Digestion of IgG by papain results in generation of fragment antigen binding (Fab) comprising of one complete L chain and a variable and CH1 region of H chain. Pepsin digestion of IgG results in fragment crystallisable (fc), comprises of the H chain constant region. IgG antibody have enormous therapeutic potential and the Fc are is for the development of therapeutic antibody. Although the antibody site is located in the terminal end of the human IgG molecule (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor (autoantibodies directed to Fc), passage through placental membrane and Staphylococcus protein A binding.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice .
Sonstige Hinweise
Antibody adsorbed with bovine, equine and human serum proteins.
Physikalische Form
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.
Angaben zur Herstellung
Adsorbed to reduce background staining with bovine, horse, or human samples.
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Produkt-Auswahlhilfe.
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 2
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
M K Magnusson et al.
Cancer gene therapy, 14(5), 468-479 (2007-02-03)
In order to use adenovirus (Ad) type 5 (Ad5) for cancer gene therapy, Ad needs to be de-targeted from its native receptors and re-targeted to a tumor antigen. A limiting factor for this has been to find a ligand that
Characterization of a Monoclonal Antibody and Its Single-Chain Antibody Fragment Recognizing the Nucleoside Triphosphatase/Helicase Domain of the Hepatitis C Virus Nonstructural 3 Protein
Zhang, Z., X., et al.
Clinical and Vaccine Immunology : CVI, 7(1), 58-63 (2000)
Laszlo L P Hosszu et al.
Communications biology, 3(1), 402-402 (2020-07-31)
Prion diseases, a group of incurable, lethal neurodegenerative disorders of mammals including humans, are caused by prions, assemblies of misfolded host prion protein (PrP). A single point mutation (G127V) in human PrP prevents prion disease, however the structural basis for
Janine R Roper et al.
The Journal of biological chemistry, 280(20), 19728-19736 (2005-03-16)
Galactose metabolism is essential in bloodstream form Trypanosoma brucei and is initiated by the enzyme UDP-Glc 4'-epimerase. Here, we show that the parasite epimerase is a homodimer that can interconvert UDP-Glc and UDP-Gal but not UDP-GlcNAc and UDP-GalNAc. The epimerase
Szymon W Manka et al.
Nature chemical biology (2023-01-17)
Recent cryogenic electron microscopy (cryo-EM) studies of infectious, ex vivo, prion fibrils from hamster 263K and mouse RML prion strains revealed a similar, parallel in-register intermolecular β-sheet (PIRIBS) amyloid architecture. Rungs of the fibrils are composed of individual prion protein
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.