Anti-MSH2 Antibody, clone FE11

MSH2 is a ubiquitously expressed nuclear protein that is involved in DNA repair processes. MSH2 is active as a heterodimer of MutS alpha and beta subunits that bind DNA at mismatched strands. The MutS alpha subunit binds to single base mismatches and dinucleotide insertion-deletion loops, whereas MutS beta detects longer insertion-deletion loops. Bound MutS subunits then form complexes with MutL alpha dimers, to coordinate downstream DNA-mismatch–repair processes. Abnormal expression of MSH2 has been linked to hereditary non-polyposis colorectal cancer type 1 and Muir-Torre syndrome.

This antibody recognizes the C-terminus of MSH2.

Epitope: C-terminus

Recombinant protein corresponding to the C-terminus of human MSH2.

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Zellzyklus, DNA-Replikation & -Reparatur

Anti-MSH2 Antibody, clone FE11 is a Mouse Monoclonal Antibody for detection of MSH2 also known as DNA mismatch repair protein Msh2, hMSH2, MutS protein homolog 2 & has been validated in WB & IHC.

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected SW-480 in human normal colonic tissue and in human adenocarcinoma tissue (Thibodeau, S. N., et al. (1996). 56(21):4836-4840.).

Evaluated by Western Blot in SW-480 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected MSH2 in 10 µg of SW-480 cell lysate.

~104 kDa observed

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
SW-480 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.