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05-632

Sigma-Aldrich

Anti-14-3-3σ Antibody, clone CS112-2A8

clone CS112-2A8, Upstate®, from mouse

Synonym(e):

Stratifin

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

CS112-2A8, monoclonal

Speziesreaktivität

mouse, human, rat

Hersteller/Markenname

Upstate®

Methode(n)

immunoprecipitation (IP): suitable
western blot: suitable

Isotyp

IgG1κ

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... SFN(2810)
mouse ... Sfn(55948)
rat ... Sfn(313017)

Spezifität

Recognizes 14-3-3σ, does not cross-react with other 14-3-3 isoforms.

Immunogen

GST fusion protein corresponding to full-length human 14-3-3 sigma

Anwendung

Research Category
Zelluläre Signaltransduktion
Research Sub Category
Cytoskelettale Signalübertragung
Detect 14-3-3σ using this Anti-14-3-3σ Antibody, clone CS112-2A8 validated for use in IP & WB.

Qualität

routinely evaluated by immunoblot on RIPA lysates of EGF-stimulated A431 cells

Zielbeschreibung

30 kDa

Physikalische Form

Protein G Purified
Format: Purified
Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Lagerung und Haltbarkeit

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hinweis zur Analyse

Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

J Zha et al.
The Journal of biological chemistry, 272(39), 24101-24104 (1997-09-26)
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Journal of dairy science, 100(2), 1072-1085 (2016-12-13)
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Journal of dairy science, 105(6), 4987-5003 (2022-04-23)
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Validation of IKK beta as therapeutic target in airway inflammatory disease by adenoviral-mediated delivery of dominant-negative IKK beta to pulmonary epithelial cells.
Matthew C Catley, Joanna E Chivers, Neil S Holden, Peter J Barnes, Robert Newton
British Journal of Pharmacology null
Surinder M Soond et al.
Cell cycle (Georgetown, Tex.), 7(9), 1214-1223 (2008-04-18)
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