Skip to Content
MilliporeSigma
All Photos(1)

Documents

G005860

HPLC Analysis of Fat Soluble Vitamins on Ascentis® Express RP-Amide, Acetonitrile Mobile Phase

application for HPLC

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RP-Amide, 15 cm x 4.6 mm I.D., 2.7 μm particles (53931-U)
mobile phase: acetonitrile
flow rate: 1.5 mL/min
pressure: 1682 psi (116 bar)
column temp.: 30 °C
detector: UV, 280 nm
injection: 5 μL
sample: 0.5 mg/mL tocopherol acetate, alpha-tocopherol, delta-tocopherol and K1, 0.05 mg/mL D2, D3 and retinol acetate, 1.5 mg/mL retinol palmitate, all in ethanol

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

Analysis was conducted using standard solution, detection via UV.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Retinyl acetate, analytical standard
    DL-alpha-Tocopherol acetate, analytical standard
    Phylloquinone (K1), analytical standard
    Ergocalciferol (D2), analytical standard
    Cholecalciferol (D3), analytical standard
    (±)-α-Tocopherol, analytical standard
    δ-Tocopherol, analytical standard
    Retinol palmitate, analytical standard
    See All (8)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service