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G005446

HPLC Analysis of Spice Cannabinoids on Ascentis® Express F5

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase: [A] 50 mM ammonium formate; [B] water; [C] acetonitrile; (10:35:55, A:B:C)
flow rate: 0.6 mL/min
pressure: 4075 psi (281 bar)
column temp.: 30 °C
detector: UV, 200 nm
injection: 3 μL
sample: 100 μg/mL in 45:55 water:acetonitrile

suitability

application for HPLC

application(s)

forensics and toxicology

Analysis Note

Synthetic cannabinoids (e.g. "Spice") are a type of designer drug that provide a cannabis-type high. New synthetic cannabinoids are continually being introduced as suppliers tweak the molecular structures. The ability to rapidly and reliably identify the continually changing population of these compounds is a significant analytical challenge facing forensic chemists. A rapid separation of eight of these compounds on Ascentis Express F5 column is shown here. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    JWH-073 N-(3-hydroxybutyl) metabolite solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
    HU-210 solution, 100 μg/mL in methanol, (Spice Cannabinoid), ampule of 1 mL, certified reference material, Cerilliant®
    JWH-018 solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
    JWH-073 solution, 100 μg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®
    Spice Cannabinoid Mix solution, 100 μg/mL each component in acetonitrile, ampule of 1.0 mL, certified reference material, Cerilliant®

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