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Supelco

Ascentis® Express 90 Å Biphenyl (2.7 µm) HPLC Columns

L × I.D. 10 cm × 2.1 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

product name

Ascentis® Express Biphenyl Column, 2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

material

stainless steel hardware

Quality Level

agency

suitable for USP L11

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

60 °C max. temp.
9000 psi max. pressure (600 bar)

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

10 cm × 2.1 mm

surface area

135 m2/g

impurities

<5 ppm metals

matrix

particle platform (Fused-Core)
superficially porous particle

matrix active group

biphenyl phase

particle size

2.7 μm

pore size

90 Å pore size

operating pH

2-9

application(s)

food and beverages

separation technique

reversed phase

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General description

The Ascentis® Express biphenyl phase chemistry offers alternative selectivity for pharmaceutical analytes and drug metabolites that are not well retained or difficult to resolve on traditional alkyl (C18) and phenyl bonded phases. Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 ?m particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UHPLC users, as well as conventional HPLC users. Visit the Ascentis Express home page for more information on this new column technology.

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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Articles

An online SPE-LC/MS method has been developed for the rapid detection of thyroid hormones in human serum with minimal hands on effort and time-consuming steps.

Comparative analysis of Supel™ BioSPME 96-Pin device with a rapid equilibrium dialysis technique for accuracy of measured values, sample cleanliness, and workflow time in drug protein binding.

Tetrahydrocannabinolic acid A solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material.

The bonded, densely endcapped, dimethyl-biphenyl stationary phase of Ascentis® Express 90 Å Biphenyl, based on fused-core particles, provides a stable, reversed phase packing with enhanced π-π and mild steric interactions due to the two sequential phenyl groups bonded to the base silica.

Protocols

Potency testing in marijuana-infused edibles is an important problem that analytical labs are facing due to the complexity of the involved matrices. Concentration of active ingredients in these edibles can range from a few parts per million to 3.5 parts per thousand. This application demonstrates the extraction and HPLC-UV analysis of the active compounds.

Thyroid hormones play critical roles in the regulation of biological processes, such as growth, metabolism, protein synthesis, and brain development. For a clinical laboratory, fast and reliable measurements of total hormone levels are important for the diagnosis and monitoring of thyroid diseases.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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