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U0508

Sigma-Aldrich

Monoclonal Anti-Ubiquitin antibody produced in mouse

clone 6C1, ascites fluid

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About This Item

MDL number:
MFCD03792436
UNSPSC Code:
12352203

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

6C1, monoclonal

contains

15 mM sodium azide

species reactivity

human, rat, mouse, bovine

technique(s)

indirect ELISA: suitable
microarray: suitable
western blot: 1:1,000 using whole cell lysate of human promyelocitic HL60 cells

isotype

IgG2a

UniProt accession no.

P0CG47

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... UBB(7314) , UBC(7316)
mouse ... Ubb(22187) , Ubc(22190)
rat ... Ubb(192255) , Ubc(50522)

General description

Ubiquitin, an 8.5kD (approx.) 76 amino acid containing protein, is highly conserved and found in both prokaryotes and eukaryotes. It facilitates protein degradation in ubiquitin/proteasome pathway and also has pivotal role in intracellular regulation. Monoclonal anti-ubiquitin antibody can be used in immunoblotting and ELISA to localize and detect ubiquitin. Mouse anti-ubiquitin antibody reacts specifically with ubiquitin in mouse, human, bovine and rat.

Immunogen

purified bovine ubiquitin conjugated to KLH.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Monoclonal anti-ubiquitin antibody can be used in western blot to visualize and evaluate ubiquitylated proteins.It can also be used in microarray and immunoblotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yu Wang et al.
Autophagy, 11(11), 2033-2047 (2015-12-10)
Autophagy plays critical roles in plant responses to stress. In contrast to the wealth of information concerning the core process of plant autophagosome assembly, our understanding of the regulation of autophagy is limited. In this study, we demonstrated that transcription
Kalidou Ndiaye et al.
Journal of ovarian research, 8, 12-12 (2015-04-18)
LAPTM4B is a member of the lysosome-associated transmembrane protein superfamily that is differentially expressed in normal human tissues and upregulated in various types of carcinomas. These proteins are thought to be involved in the regulation of cell proliferation and survival.
Clinton Yu et al.
Nature communications, 6, 10053-10053 (2015-12-04)
The full enzymatic activity of the cullin-RING ubiquitin ligases (CRLs) requires a ubiquitin-like protein (that is, Nedd8) modification. By deamidating Gln40 of Nedd8 to glutamate (Q40E), the bacterial cycle-inhibiting factor (Cif) family is able to inhibit CRL E3 activities, thereby
Sarita Lagalwar et al.
Acta neuropathologica, 113(1), 63-73 (2006-11-08)
Protein misfolding is a distinguishing feature of a number of neurodegenerative diseases. Accumulation of misfolded protein often results in cellular lesions, the location of lesions correlating with the nature of symptoms. Alzheimer's disease (AD), Progressive Supranuclear Palsy (PSP), Corticobasal Degeneration
Andrej Susor et al.
Reproduction (Cambridge, England), 134(4), 559-568 (2007-09-25)
In this study, we performed proteomic analysis of porcine oocytes during in vitro maturation. Comparison of oocytes at the initial and final stages of meiotic division characterized candidate proteins that were differentially synthesized during in vitro maturation. While the biosynthesis
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