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T9568

Sigma-Aldrich

Trizma® Pre-set crystals

BioPerformance Certified, pH 8.9, average Mw 125.6

Synonym(s):

Tris crystals

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

grade

BioPerformance Certified

Quality Level

200

form

crystalline powder

mol wt

average Mw 125.6

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, NICKase, none detected
 endotoxin, tested
 total aerobic microbial count, tested
≤0.05 A290
≤0.5% water (Karl Fischer)
≤5 ppm Heavy metals (as Pb)

pH

8.90 (25 °C, 0.1 M)(lit.)
8.9

useful pH range

7.0-9.0

solubility

water: 0.66 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

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Application

Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.
The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.

Other Notes

This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer.
Certain pH electrodes do not give accurate results with Tris buffers.

Preparation Note

Trizma solutions can be autoclaved.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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