SRP5232
EIF2S1, His tagged human
recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
EIF-2, EIF-2α, EIF-2A, EIF2, EIF2A
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About This Item
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biological source
human
recombinant
expressed in E. coli
assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
mol wt
~40 kDa
NCBI accession no.
application(s)
cell analysis
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... EIF2S1(1965)
General description
Eukaryotic translation initiation factor 2 subunit 1 α (EIF2S1) is a 35kDa protein. It is the α subunit of the eukaryotic initiation factor 2 (eIF2α). The gene encoding it is localized on human chromosome 14q23.3.
Biochem/physiol Actions
Eukaryotic translation initiation factor 2 subunit 1 α (EIF2S1) catalyzes the first regulated step of protein synthesis initiation and promotes the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. The Ser51 in the mature human EIF2-α protein acts as the sole site of phosphorylation that leads to repression of protein synthesis. EIF2-α also plays an essential role in erythropoiesis.
Physical form
Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Direct interaction between STAT3 and EIF2AK2 controls fatty acid-induced autophagy
Mireia N
Autophagy (2012)
V K Pathak et al.
Molecular and cellular biology, 8(2), 993-995 (1988-02-01)
The phosphorylation of the alpha-subunit of initiation factor eIF-2 leads to an inhibition of protein synthesis in mammalian cells. We have performed site-directed mutagenesis on a cDNA encoding the alpha-subunit of human eIF-2 and have replaced the candidate sites of
A SNP microarray and FISH-based procedure to detect allelic imbalances in multiple myeloma: An integrated genomics approach reveals a wide gene dosage effect
Luca A
Genes (2009)
Ppp1r15 gene knockout reveals an essential role for translation initiation factor 2 alpha (eIF2a) dephosphorylation in mammalian development
Heather P
Proceedings of the National Academy of Sciences of the USA (2008)
ER stress and autophagy contribute to CsA-induced death of malignant glioma cells
Iwona Anna
Autophagy (2012)
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