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SRP3238

Sigma-Aldrich

EGF from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

Epidermal Growth Factor, URG, Urogastrone

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

rat

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤0.1 ng/mL

mol wt

6.2 kDa

packaging

pkg of 100 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

P07522

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... EGF(25313)

Related Categories

General description

Epidermal growth factor (EGF) is a potent growth factor and a cytoprotective peptide. Recombinant rat EGF is a 6.2kDa globular protein containing 54 amino acid residues, including 3 intramolecular disulfide-bonds.

Application

Epidermal growth factor (EGF) is used in hepatocyte culture to determine the optimal condition for survival and function of rat hepatocytes.

Biochem/physiol Actions

Epidermal growth factor (EGF) stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, inflammation and is involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α (transforming growth factor-α) and VGF (vaccinia virus growth factor).

Sequence

MNSNTGCPPS YDGYCLNGGV CMYVESVDRY VCNCVIGYIG ERCQHRDLRW WKLR

Physical form

Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The role of epidermal growth factor in prevention of oxidative injury and apoptosis induced by intestinal ischemia/reperfusion in rats.
Arda-Pirinci P
Acta Histochemica, 116(1), 167-175 (2014)
Epidermal growth factor improves survival and prevents intestinal injury in a murine model of pseudomonas aeruginosa pneumonia.
Dominguez JA
Shock, 36(4), 381-389 (2011)
The epidermal growth factor receptor family: biology driving targeted therapeutics.
Wieduwilt MJ and Moasser MM
Cellular and Molecular Life Sciences, 65(10), 1566-1584 (2008)
Florence E Perrin et al.
PloS one, 5(12), e15914-e15914 (2011-01-07)
Spinal cord injury (SCI) is a widely spread pathology with currently no effective treatment for any symptom. Regenerative medicine through cell transplantation is a very attractive strategy and may be used in different non-exclusive ways to promote functional recovery. We
Epidermal growth factor.
G Carpenter et al.
The Journal of biological chemistry, 265(14), 7709-7712 (1990-05-15)
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