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SAB5900203

Alpaca Anti-hTNFR, recombinant single domain Antibody

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Recombinant Alpaca(VHH) Nano antibody

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About This Item

UNSPSC Code:
12352203

biological source

alpaca

Quality Level

100

recombinant

expressed in (E. coli)

conjugate

unconjugated

antibody form

(Affinity purified VHH antibody)

clone

TR09, monoclonal

assay

>95% (SDS-PAGE)

form

lyophilized solid

mol wt

14.2 kDa
 ~15-18 kDa (SDS PAGE under reducing condition)

purified by

affinity chromatography (purified via Ni-charged resin)

species reactivity

human

concentration

1 mg/mL

technique(s)

ELISA: 1:1000-1:10000
immunofluorescence: 1:100-1:300
immunohistochemistry: 1:100-1:300
western blot: 1:500-1:3000

color

colorless

pH

( in PBS, pH 7.4)

isotype

VHH

Protein ID accession no.

NP_001056.1

UniProt accession no.

P19438

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... TNFRSF1A(7132)

General description

Tumor necrosis factor receptor 1 (TNFR1), also known as tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) and CD120a (cluste of differentiation 120a), is an ubiquitous membrane receptor that binds tumor necrosis factor-alpha (TNFα). It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). TNFR1 can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.

Specificity

Human Tumor necrosis factor receptor 1 (TNFR1) / CD120a

Immunogen

Recombinant human TNFR1 (Ile22-Thr211) protein

Application

ELISA, WB, IHC, IF

Packaging

50 µg per vial / 100 µg per vial

Physical form

Purified VHH protein in buffer containing 25 mM TAPS pH8.5, 500 mM NaCl, 5 mM EDTA

Reconstitution

Reconstitute the lyophilized protein with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

Storage and Stability

Stable for 1 years at 4°C from date of receipt.

Other Notes

Storage at 4°C; Do not freeze.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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