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SAB5500057

Sigma-Aldrich

Anti-CD3 antibody, Rabbit monoclonal

recombinant, expressed in proprietary host, clone SP162, affinity isolated antibody

Synonym(s):

Anti-CD3epsilon, Anti-IMD18, Anti-T3E, Anti-TCRE

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

recombinant

expressed in proprietary host

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

SP162, monoclonal

species reactivity

human (tested)

species reactivity (predicted by homology)

rat, chicken, rabbit, mouse, pig, bovine, dog

technique(s)

flow cytometry: 1:150
immunoblotting: 1:150
immunohistochemistry: 1:150

isotype

IgG

UniProt accession no.

P07766

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... CD3E(916)

Related Categories

General description

CD3E (CD3e molecule) gene is mapped to human chromosome 11q23.3. This gene encodes fora subunit of T-cell regulator (TCR) protein complex. It possesses IgG (immunoglobulin G)-like extracelluar domain at the N-terminal, a transmembrane domain, a peptide bridge and an immunoreceptor tyrosine-based activation motif (ITAM) towards the cytoplasm. The protein also contains basic residue-rich sequence (BRS).
This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues. This antibody is suitable for staining normal and neoplastic T cells in formalin-fixed, paraffin-embedded tissues.

Immunogen

Synthetic peptide corresponding to internal region of the epsilon chain of human CD3 protein.

Application

Anti-CD3 antibody, Rabbit monoclonal has been used in immunohistochemistry.

Biochem/physiol Actions

CD3E (CD3e molecule) gene is associated with actin cytoskeletal rearrangement, through activation of a number of signaling pathways. CD3E is essential for membrane binding of T-cell regulator (TCR) protein complex.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

0.1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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