SAB4200785
Anti-B2M antibody, Mouse monoclonal
clone 92A, purified from hybridoma cell culture
Synonym(s):
Anti-Beta-2-microglobulin
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
92A, monoclonal
form
buffered aqueous solution
mol wt
~15 kDa
species reactivity
human
concentration
~1 mg/mL
technique(s)
immunoblotting: 0.1-0.2 μg/mL using β2-Microglobulin from human urine.
immunoprecipitation (IP): 2.5-5 μg/test using lysate of human HeLa cell line.
indirect ELISA: 0.2-0.4 μg/mL using 1 μg/ml β2-Microglobulin from human urine for coating.
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... B2M(567)
General description
B2M (β2-Microglobulin) is the light chain of the major histocompatibility class (MHC) I molecule expressed on the cell surface of all nucleated cells, including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells and is absent in erythrocytes. B2M is filtered during glomerular filtrations and re-absorbed and catabolized by proximal renal tubule. While under normal conditions the B2M is undetectable in urine, its increased urinary excretion has been observed to be an early marker of tubular injury in a number of settings, including nephrotoxicant exposure, cardiac surgery and renal transplantation, preceding rises in serum creatinine by as many as 4-5 days. B2M may also serve as an early biomarker for AKI (Acute Kidney Injury). B2M plays a vital role in cell survival, proliferation and metastasis in various types of cancer. Its levels are also linked with multiple myeloma (MM) and non-Hodgkin′s lymphoma (NHL). NHL patients showing higher levels of B2M have a poor disease prognosis and a higher mortality risk. The protein has a predominantly β-pleated sheet structure that can form amyloid fibrils in some pathological conditions. In patient subjected to long-term hemodialysis, B2M is responsible for systemic amyloidosis. The levels of this protein are also altered in the cerebrospinal fluid of patients with neurological diseases, such as leptomeningeal metastasis, purulent meningitis, viral meningitis or encephalitis and neuroborreliosis. Over-expression of serum B2M is observed in hepatitis C virus (HCV) related chronic liver diseases and thus acts as a potential marker for the HCV disease progression towards cirrhosis and carcinoma.
Immunogen
Recombinant B2M protein.
Application
Immunoblotting: a working concentration of 0.1-0.2 μg/mL is recommended using β2-Microglobulin from human urine.
Immunoprecipitation: a working concentration of 2.5-5 μg/test is recommended using lysate of human HeLa cell line.
Indirect ELISA: a working concentration of 0.2-0.4 μg/mL is recommended using 1 μg/mL ß2-Microglobulin from human urine for coating.
Immunoprecipitation: a working concentration of 2.5-5 μg/test is recommended using lysate of human HeLa cell line.
Indirect ELISA: a working concentration of 0.2-0.4 μg/mL is recommended using 1 μg/mL ß2-Microglobulin from human urine for coating.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15mM sodium azide.
Other Notes
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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M C Chapelsky et al.
Drug safety, 7(4), 304-309 (1992-07-01)
This study assessed the potential nephrotoxicity of clarithromycin in comparison with gentamicin and placebo. Increased urinary excretion of alanine aminopeptidase (AAP) and N-acetyl-beta-D-glucosaminidase (NAG) served as markers of renal tubular injury. The study utilised a multiple-dose, double-blind, randomised, parallel group
N E Tolkoff-Rubin et al.
Clinics in laboratory medicine, 8(3), 507-526 (1988-09-01)
Traditional methods of noninvasively evaluating patients for renal injury do not accomplish the following tasks: reliably distinguish potentially treatable forms of acute renal failure from acute tubular necrosis; provide a sensitive indicator of early allograft rejection in renal transplant recipients
Philip R Greipp et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 23(15), 3412-3420 (2005-04-06)
There is a need for a simple, reliable staging system for multiple myeloma that can be applied internationally for patient classification and stratification. Clinical and laboratory data were gathered on 10,750 previously untreated symptomatic myeloma patients from 17 institutions, including
Stefan Schaub et al.
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 5(4 Pt 1), 729-738 (2005-03-12)
Our aim is to develop noninvasive tests to monitor the renal allograft posttransplant. Previously, we have reported that an unbiased proteomic-based approach can detect urine protein peaks associated with acute tubulointerstitial renal allograft rejection. Identification of these proteins peaks by
M G Dehne et al.
Der Anaesthesist, 44(8), 545-551 (1995-08-01)
After cardiac surgery, transient renal dysfunction often occurs. Regional differentiation of these processes is possible only using invasive techniques, including renal biopsy. Approximately 30 different plasma protein components have been identified in the urine of healthy individuals by means of
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