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SAB4200347

Sigma-Aldrich

Anti-Nestin (C-terminal region) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

Synonym(s):

Anti-Nbla00170

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~300 kDa

species reactivity

rat, human, mouse

concentration

~1.5 mg/mL

technique(s)

indirect immunofluorescence: 1-2 μg/mL using mouse P19 cells.
western blot: 2-4 μg/mL using lysates of HEK293T cells over-expressing rat nestin.

UniProt accession no.

P48681

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NES(10763)
mouse ... Nes(18008)
rat ... Nes(25491)

Related Categories

General description

Nestin (NES) is a class VI cytoplasmic protein which is expressed in the intermediate filaments. It has a molecular weight of 177.4kDa. The gene encoding it is localized on human chromosome 1q23.1 and has four exons.
Nestin is expressed during early stages of development in the central nervous system (CNS) and in myogenesis. Nestin is found in neurogenic progenitor cells (NPCs) and has been used as a marker for neural progenitor cells.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human nestin, conjugated to KLH. The sequence is highly conserved (72% identity) in rat and mouse nestin.

Application

Anti-Nestin (C-terminal region) antibody has been used in
  • immunofluorescence
  • Proximity ligation assay
  • immunohistofluorescence staining
  • immunofluorescence labeling
  • immunoblotting

Anti-Nestin (C-terminal region) antibody produced in rabbit has been used in western blotting and immunostaining.

Biochem/physiol Actions

Nestin (NES) functions as a modulator of proteins which are involved in cell growth. It also interacts with other proteins of the intermediate filament. NES has been studied as a marker of neural stem cells.
Nestin expression is down-regulated and replaced by the expression of other intermediate filament proteins such as neurofilament and Glial fibrillary acidic protein (GFAP). Nestin expression is re-induced in the adult organism during pathological conditions, such as formation of glial scar after injury to the central nervous system (CNS) and in certain types of CNS tumors. Nestin has been shown to play an important role in the phosphorylation-dependent disassembly of vimentin intermediate filaments during mitosis and is a potential target for Cyclin-dependent kinase 5 (cdk5) and p35 during development.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

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Suppression of Nestin reveals a critical role for p38-EGFR pathway in neural progenitor cell proliferation
Hu W, et al.
Testing, 7(52), 87052-87052 (2016)
Scutellarin regulates microglia-mediated TNC1 astrocytic reaction and astrogliosis in cerebral ischemia in the adult rats.
Fang M
BMC Neuroscience (2015)
Nestin expression in hair follicle sheath progenitor cells
Li L, et al.
Proceedings of the National Academy of Sciences of the USA, 100(17), 9958-9961 (2003)
Nestin promotes the phosphorylation-dependent disassembly of vimentin intermediate filaments during mitosis
Chou Y, et al.
Molecular Biology of the Cell, 14(4), 1468-1478 (2003)
The Structural Function of Nestin in Cell Body Softening is Correlated with Cancer Cell Metastasis
Yamagishi A, et al.
International Journal of Biological Sciences, 15(7), 1546-1546 (2019)
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