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S2822

Sigma-Aldrich

Anti-Synapsin II antibody produced in rabbit

enhanced validation

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-SYN2, Anti-SYNII, Anti-SYNIIa, Anti-SYNIIb, Anti-Synapsin 2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~54 kDa (synapsin IIb)
antigen ~74 kDa (synapsin IIa)

species reactivity

mouse, human, rat

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1 mg/mL

technique(s)

western blot: 0.5-1 μg/mL using extracts of HEK-293T cells expressing recombinant human synapsin II, mouse brain extract (S1 fraction), and rat PC12 cell lysate

UniProt accession no.

Q92777

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SYN2(6854)
mouse ... Syn2(20965)
rat ... Syn2(29179)

Related Categories

General description

Synapsin II (SYN-2) is a neuronal phosphoprotein present in synaptic vesicles. The SYN-2 gene is mapped to human chromosome 3p25.2. Structurally, synapsin types have a conserved N -terminal region and vary in their C-terminal region.

Specificity

Anti-Synapsin II specifically recognizes human, rat, and mouse synapsin IIa and Iib.

Immunogen

synthetic peptide corresponding to amino acids 438-453 located near the C-terminus of rat synapsin II, conjugated to KLH. This sequence is identical in rat synapsin IIa and IIb isoforms and mouse synapsin II, and is highly conserved in human synapsin IIa and IIb (one amino acid substitution).

Application

Anti-Synapsin II antibody produced in rabbit may be used in immunoblotting.

Biochem/physiol Actions

Synapsins regulate synaptic vesicle clustering, neurotransmitter release, and the formation of synaptic terminals. Synapsin II is involved in the formation of synaptic terminals during neuronal development. Similar to synapsin I, synapsin II can link synaptic vesicles to the actin cytoskeleton, thus regulating the availability of synaptic vesicles for exocytosis. Synapsin II association with synaptic vesicles is controlled by phosphorylation. Synapsin II is proposed as a candidate associated with increased vulnerability to schizophrenia. It is also implicated in synaptogenesis and neurotransmitter release impairment in multiple neuropsychiatric diseases.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Qi Chen et al.
American journal of human genetics, 75(5), 873-877 (2004-09-28)
Synapsin II has been proposed as a candidate gene for vulnerability to schizophrenia on the basis of its function and its location in a region of the genome implicated by linkage studies in families with schizophrenia. We recently reported positive
Seong-Yeon Bae et al.
Experimental neurobiology, 26(2), 82-89 (2017-04-27)
Translationally controlled tumor protein (TCTP) is a cytosolic protein with microtubule stabilization and calcium-binding activities. TCTP is expressed in most organs including the nervous system. However, detailed distribution and functional significance of TCTP in the brain remain unexplored. In this
A Ferreira et al.
Molecular medicine (Cambridge, Mass.), 4(1), 22-28 (1998-03-26)
The synapsins are a family of neuron-specific proteins, associated with the cytoplasmic surface of synaptic vesicles, which have been shown to regulate neurotransmitter release in mature synapses and to accelerate development of the nervous system. Using neuronal cultures from mice

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