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P6186

Sigma-Aldrich

Thidiazuron

BioReagent, suitable for plant cell culture

Synonym(s):

1-Phenyl-3-(1,2,3-thiadiazol-5-yl)urea, TDZ

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About This Item

Empirical Formula (Hill Notation):
C9H8N4OS
CAS Number:
51707-55-2
Molecular Weight:
220.25
Beilstein/REAXYS Number:
1078092
EC Number:
257-356-7
MDL number:
MFCD00078723
UNSPSC Code:
10171502
PubChem Substance ID:
24898652
NACRES:
NA.72

product line

BioReagent

Quality Level

200

form

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

SMILES string

O=C(Nc1ccccc1)Nc2cnns2

InChI

1S/C9H8N4OS/c14-9(12-8-6-10-13-15-8)11-7-4-2-1-3-5-7/h1-6H,(H2,11,12,14)

InChI key

HFCYZXMHUIHAQI-UHFFFAOYSA-N

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Application

Thidiazuron has been used:
  • as a component of the proliferation medium to test its effect on the proliferation of embryo-like structures obtained from the post-culture of shoot tips
  • as a growth regulator in Murashige and Skoog (MS) media to culture the nodal segments of Hyssopus officinalis L. seedlings
  • as a cytokinin in induction media to experiment with the hormonal composition

Biochem/physiol Actions

Thidiazuron (TDZ) is a plant growth regulator and a synthetic diphenylurea cytokinin used in micropropagations to supplement media such as Murashige and Skoog medium. TDZ promotes plant organogenesis (shoot regeneration) and plant regeneration. Thidiazuron (TDZ) enables the production of virus-free stock plants, speeds up breeding, and provides new genotypes for the market. It is also used as a phenylurea herbicide for controlling weeds in agricultural and nonagricultural practices. Thidiazuron acts as a pesticide by inhibiting photosystem II.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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María Laura Fontana et al.
Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al, 33(3), 179-186 (2010-01-14)
With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: alpha-naphthalenacetic
Wanwimon Lualon et al.
Zeitschrift fur Naturforschung. C, Journal of biosciences, 63(1-2), 96-100 (2008-04-05)
An efficient in vitro method for multiple shoot bud induction and regeneration has been developed in Artemisia annua L. using leaf and stem explants in various concentrations and combinations of plant growth regulators to evaluate the frequency of regeneration. The
Sonia Malik et al.
Cell biology international, 34(5), 537-542 (2010-01-09)
The present study highlights the importance of preculture time and concentration of TDZ (thidiazuron) for direct regeneration from in vitro leaves (attached to shoots) in Arnebia euchroma. Shoot buds proliferated to form multiple shoots on MS medium (Murashige and Skoog
María A Alvarez et al.
Biotechnology letters, 31(3), 457-463 (2008-11-04)
In vitro cultures of Berberis buxifolia were established using thidiazuron (4.5, 23 and 45 mM) or picloram (4 and 40 mM) as plant growth regulators for sustaining growth. For producing berberine, a two-stage culture was performed. In the first step
Ji-Ye Hu et al.
Bulletin of environmental contamination and toxicology, 87(4), 448-451 (2011-08-13)
A rapid, sensitive and reliable analytical method for thidiazuron residues in apple and soil was established. The residual levels of the pesticide in apple and soil were determined by high performance liquid chromatography (HPLC) with UV detector. Samples of apple
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