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P3118

Sigma-Aldrich

Monoclonal Anti-p115/TAP antibody produced in mouse

clone 5D6, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-Transcytosis Associated Protein

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About This Item

MDL number:
MFCD03792426
UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5D6, monoclonal

form

buffered aqueous solution

species reactivity

rat

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 2-4 μg/mL using cytosolic and membrane extracts of rat liver

isotype

IgG1

UniProt accession no.

P41542

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

rat ... Uso1(56042)

General description

Monoclonal Anti-p115/TAP (mouse IgG1 isotype) is derived from the 5D6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice. p115, a Golgin family tether is also referred as TAP1 (transcytosis associated protein). It is homologous to USO1 vesicle transport factor (Uso1p). It has a coiled coil dimerization domain, an N-terminal globular domain and a C-terminal acidic domain.

Specificity

Monoclonal Anti-p115/TAP recognizes rat p115/TAP (approx. 95-100 kDa).

Immunogen

purified rat p115/TAP.

Application

Monoclonal Anti-p115/TAP antibody produced in mouse may be used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry

Biochem/physiol Actions

p115/TAP1 (transcytosis associated protein) plays an important role in endoplasmic reticulum to Golgi traffic and in intra−Golgi traffic. It is known to play a key role in the transport of endoplasmic reticulum (ER)/Golgi. p115 is highly essential for the docking or tethering step in intra-Golgi vesicular transport and transcytosis. It serve as a catalyst during the fusion mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

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On and off membrane dynamics of the endoplasmic reticulum-Golgi tethering factor p115 in vivo
Brandon E, et al.
Molecular Biology of the Cell, 17(7), 2996-3008 (2006)
M Barroso et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(2), 527-531 (1995-01-17)
Transcytosis-associated protein (TAP) is found on transytotic vesicles (TCVs) and is required for their fusion with the target membrane. We developed a cell-free assay capable of differentiating targeting/binding of TCVs to membrane from later fusion events. We found that TAP
Gabriella Frigerio et al.
Traffic (Copenhagen, Denmark), 8(11), 1644-1655 (2007-09-01)
ADP-ribosylation factors (ARFs) are critical regulators of vesicular trafficking pathways and act at multiple intracellular sites. ADP-ribosylation factor-GTPase-activating proteins (ARFGAPs) are proposed to contribute to site-specific regulation. In yeast, two distinct proteins, Glo3p and Gcs1p, together provide overlapping, essential ARFGAP

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