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N5665

Sigma-Aldrich

BLOCKED P-NITROPHENYL-ALPHA-D-MALTOHEPTAOSIDE

Synonym(s):

BpNPG7

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About This Item

UNSPSC Code:
12352200

form

powder

Quality Level

mol wt

1362

impurities

≤3% Water content (Karl Fischer)

color

white to yellow

shipped in

dry ice

storage temp.

−20°C

General description

This product is useful as a substrate for determination of α-amylase activity. This oligosaccharide contains a blocking group at the non-reducing end, which prevents exoglucosidase from attacking the substrate molecule. Amylase can still cleave the substrate internally, which releases short p-nitrophenol-linked (pNP-linked) oligosaccharides. After removal of the blocking group, auxiliary enzymes such as glucoamylase and maltase can release the chromogen. Glucoamylase accelerates this reaction via action on the longer chains. Maltase then separates these longer chains from the pNP moiety.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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DNA typing after α-amylase test.
Rapone, C. et al.
International Congress Series, 1239, 911-915 (2003)

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