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N3643

Sigma-Aldrich

NZCYM Broth

Powder microbial growth medium

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.85

grade

for molecular biology

Quality Level

200

sterility

non-sterile

form

powder

composition

Casamino Acids, 1 g/L
MgSO4, 0.98 g/L
NaCL, 5 g/L
Tryptone, 10 g/L
Yeast extract, 5 g/L

technique(s)

microbiological culture: suitable

pH

6.8-7.2(2.2% solution)

application(s)

food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

Related Categories

General description

NZCYM is a microbial growth medium used for the cultivation of E. coli and lambda bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Application

NZCYM Broth has been used for non-selective cultivation of lambda, MS2, T7, CLP-P3, Phi29, and Goe1 bacteriophages, and E. coli strains for cloning, DNA plasmid production, and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

NZCYM powder provides:
  • Standard formulation
  • Convenient package sizes
  • A budget-friendly alternative to liquid

Reconstitution

Stir to suspend 22 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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